A Set of Lotus japonicus Gifu x Lotus burttii Recombinant Inbred Lines Facilitates Map-based Cloning and QTL Mapping

Model legumes such as Lotus japonicus have contributed significantly to the understanding of symbiotic nitrogen fixation. This insight is mainly a result of forward genetic screens followed by map-based cloning to identify causal alleles. The L. japonicus ecotype 'Gifu' was used as a commo...

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Bibliographic Details
Published in:DNA research 2012-08, Vol.19 (4), p.317-323
Main Authors: SANDAL, Niels, JIN, Haojie, RUIZ-SAINZ, Jose E, ANDERSEN, Stig U, STOUGAARD, Jens, RODRIGUEZ-NAVARRO, Dulce Nombre, TEMPRANO, Francisco, CVITANICH, Cristina, BRACHMANN, Andreas, SATO, Shusei, KAWAGUCHI, Iviasayoshi, TABATA, Satoshi, PARNISKE, Martin
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chromosome Mapping
Crosses, Genetic
Ecotype
Fundamental and applied biological sciences. Psychology
Genes, Plant
Genes. Genome
Genetic Linkage
Genetics of eukaryotes. Biological and molecular evolution
Genotype
Loteae - genetics
Molecular and cellular biology
Molecular genetics
Quantitative Trait Loci
Recombination, Genetic
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Summary:Model legumes such as Lotus japonicus have contributed significantly to the understanding of symbiotic nitrogen fixation. This insight is mainly a result of forward genetic screens followed by map-based cloning to identify causal alleles. The L. japonicus ecotype 'Gifu' was used as a common parent for inter-accession crosses to produce F2 mapping populations either with other L. japonicus ecotypes, MG-20 and Funakura, or with the related species L. filicaulis. These populations have all been used for genetic studies but segregation distortion, suppression of recombination, low polymorphism levels, and poor viability have also been observed. More recently, the diploid species L. burttii has been identified as a fertile crossing partner of L. japonicus. To assess its qualities in genetic linkage analysis and to enable quantitative trait locus (QTL) mapping for a wider range of traits in Lotus species, we have generated and genotyped a set of 163 Gifu × L. burttii recombinant inbred lines (RILs). By direct comparisons of RIL and F2 population data, we show that L. burttii is a valid alternative to MG-20 as a Gifu mapping partner. In addition, we demonstrate the utility of the Gifu × L. burttii RILs in QTL mapping by identifying an Nfr1-linked QTL for Sinorhizobium fredii nodulation.
ISSN:1340-2838
1756-1663
DOI:10.1093/dnares/dss014