Validation of an enzyme immunoassay for the measurement of faecal glucocorticoid metabolites in spotted hyenas ( Crocuta crocuta )

Highlights ► We validated a cortisol-3-CMO enzyme immunoassay (EIA) for spotted hyenas. ► The EIA detected several cortisol metabolites produced by spotted hyenas. ► The EIA measured elevated fGCM levels after ACTH challenge and anaesthesia. ► When young, juveniles experience a more unpredictable en...

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Bibliographic Details
Published in:General and comparative endocrinology 2012-09, Vol.178 (2), p.265-271
Main Authors: Benhaiem, Sarah, Dehnhard, Martin, Bonanni, Roberto, Hofer, Heribert, Goymann, Wolfgang, Eulenberger, Klaus, East, Marion L
Format: Article
Language:English
Subjects:
ACTH
age
Age effect
anesthesia
Animals
captive animals
corticosterone
corticotropin
cortisol
Crocuta crocuta
Endocrinology & Metabolism
Enzyme immunoassay
enzyme immunoassays
Faecal glucocorticoid metabolites
Feces - chemistry
Female
Glucocorticoids - analysis
high performance liquid chromatography
Hyaenidae
Immunoenzyme Techniques - methods
juveniles
Male
metabolites
Radiolabelled metabolites
Spotted hyena
vertebrates
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Summary:Highlights ► We validated a cortisol-3-CMO enzyme immunoassay (EIA) for spotted hyenas. ► The EIA detected several cortisol metabolites produced by spotted hyenas. ► The EIA measured elevated fGCM levels after ACTH challenge and anaesthesia. ► When young, juveniles experience a more unpredictable environment than when older. ► When young, juveniles had higher fGCM levels than when older.
ISSN:0016-6480
1095-6840
DOI:10.1016/j.ygcen.2012.05.006