High-Performance Graphene-Titania Platform for Detection of Phosphopeptides in Cancer Cells

Phosphopeptides play a crucial role in many biological processes and constitute some of the most powerful biomarkers in disease detection. However they are often present in very low concentration, which makes their detection highly challenging. Here, we demonstrate the use of a solution-dispersible...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2012-08, Vol.84 (15), p.6693-6700
Main Authors: Tang, Lena Ai Ling, Wang, Junzhong, Lim, Teck Kwang, Bi, Xuezhi, Lee, Wong Cheng, Lin, Qingsong, Chang, Young-Tae, Lim, Chwee Teck, Loh, Kian Ping
Format: Article
Language:English
Subjects:
Biomarkers
Cancer
Cells
Computational Biology
Desorption
Graphite - chemistry
HeLa Cells
Humans
Isoelectric Point
Mass spectrometry
Nanotechnology
Peptides
Phosphopeptides - analysis
Solutions - chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Titanium - chemistry
Trypsin - metabolism
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Summary:Phosphopeptides play a crucial role in many biological processes and constitute some of the most powerful biomarkers in disease detection. However they are often present in very low concentration, which makes their detection highly challenging. Here, we demonstrate the use of a solution-dispersible graphene-titania platform for the selective extraction of phosphopeptides from peptide mixtures. This is followed by direct analysis by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). The efficient charge and energy exchange between graphene and TiO2 during laser irradiation in SELDI-TOF MS promotes the soft ionization of analytes and affords a detection limit in the attomole range, which is 102–105 more sensitive than conventional platforms. The graphene-titania platform can also be used for detecting phosphopeptides in cancer cells (HeLa cells), where it shows high specificity (94%). An expanded library of 967 unique phosphopeptides is detected using significantly reduced loading of extraction matrixes compared to conventional TiO2 bead-based assays.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac301119r