Accumulation of phosphate and polyphosphate by Cryptococcus humicola and Saccharomyces cerevisiae in the absence of nitrogen

Abstract The search for new phosphate-accumulating microorganisms is of interest in connection with the problem of excess phosphate in environment. The ability of some yeast species belonging to ascomycetes and basidiomycetes for phosphate (Pi) accumulation in nitrogen-deficient medium was studied....

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Bibliographic Details
Published in:FEMS yeast research 2012-09, Vol.12 (6), p.617-624
Main Authors: Breus, Natalia A., Ryazanova, Lubov P., Dmitriev, Vladimir V., Kulakovskaya, Tatiana V., Kulaev, Igor S.
Format: Article
Language:English
Subjects:
Cryptococcus
Cryptococcus - metabolism
Cryptococcus humicola
Culture Media - chemistry
DAPI
fluorescence microscopy
Fungal infections
Glucose - metabolism
inorganic polyphosphate
Magnesium
magnesium ion
Magnesium Sulfate - metabolism
Mineralization
Nitrogen - metabolism
nitrogen starvation
phosphate uptake
Phosphorus
phosphorus‐accumulating organism
Polyphosphates - metabolism
Saccharomyces cerevisiae
Saccharomycetales - metabolism
Ustilaginales - metabolism
Yeast
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Summary:Abstract The search for new phosphate-accumulating microorganisms is of interest in connection with the problem of excess phosphate in environment. The ability of some yeast species belonging to ascomycetes and basidiomycetes for phosphate (Pi) accumulation in nitrogen-deficient medium was studied. The ascomycetous Saccharomyces cerevisiae and Kuraishia capsulata and basidiomycetous Cryptococcus humicola, Cryptococcus curvatus, and Pseudozyma fusiformata were the best in Pi removal. The cells of Cryptococcus humicola and S. cerevisiae took up 40% Pi from the media containing Pi and glucose (5 and 30 mM, respectively), and up to 80% upon addition of 5 mM MgSO4. The cells accumulated Pi mostly in the form of polyphosphate (PolyP). In the presence of Mg2+, the content of PolyP with longer average chain length increased in both yeasts; they both had numerous inclusions fluorescing in the yellow region of the spectrum, typical of DAPI-PolyP complexes. Among the yeast species tested, Cryptococcus humicola is a new promising model organisms to study phosphorus removal from the media and biomineralization in microbial cells.
ISSN:1567-1356
1567-1364
DOI:10.1111/j.1567-1364.2012.00812.x