Characterization of myofibroblasts in chronic thromboembolic pulmonary hypertension

Abstract Background It has been generally accepted that chronic thromboembolic pulmonary hypertension (CTEPH) results from pulmonary embolism arising from deep vein thrombosis. An unresolved question regarding the etiology of CTEPH is why pulmonary thromboemboli are stable and resistant to effective...

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Bibliographic Details
Published in:International journal of cardiology 2012-08, Vol.159 (2), p.119-127
Main Authors: Maruoka, Miki, Sakao, Seiichiro, Kantake, Masashi, Tanabe, Nobuhiro, Kasahara, Yasunori, Kurosu, Katsushi, Takiguchi, Yuichi, Masuda, Masahisa, Yoshino, Ichiro, Voelkel, Norbert F, Tatsumi, Koichiro
Format: Article
Language:English
Subjects:
Adult
Aged
Biological and medical sciences
Blood and lymphatic vessels
Cardiology. Vascular system
Cardiovascular
Cell Line
Cell Line, Tumor
Cells, Cultured
Chronic Disease
Coculture Techniques
Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous
Endarterectomy - methods
Female
Humans
Hypertension, Pulmonary - pathology
Hypertension, Pulmonary - surgery
Male
Medical sciences
Middle Aged
Myofibroblasts - pathology
Pneumology
Pulmonary circulation
Pulmonary Embolism - pathology
Pulmonary Embolism - surgery
Pulmonary hypertension
Pulmonary hypertension. Acute cor pulmonale. Pulmonary embolism. Pulmonary vascular diseases
Pulmonary thromboembolism
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Summary:Abstract Background It has been generally accepted that chronic thromboembolic pulmonary hypertension (CTEPH) results from pulmonary embolism arising from deep vein thrombosis. An unresolved question regarding the etiology of CTEPH is why pulmonary thromboemboli are stable and resistant to effective anticoagulation. Recently non-resolving pulmonary thromboemboli in CTEPH have been shown to include myofibroblasts. This study investigates the cellular characteristics of myofibroblasts included in the organized thrombotic tissues of CTEPH. Methods Organized thrombotic tissues of patients with CTEPH were obtained following pulmonary endarterectomy. We isolated cells from endarterectomized tissue from patients with CTEPH and identified them as endothelial-like cells and myofibroblast-like cells. Results Myofibroblast-like cells were characterized as hyperproliferative, anchorage-independent, invasive and serum-independent. Conclusions Here we report the presence of active myofibroblast-like cells in endarterectomized tissue of CTEPH. We suggest that the formation of myofibroblasts with a high growth potential in the organized thrombotic tissues may be an important event in the pathobiology of this disease.
ISSN:0167-5273
1874-1754
DOI:10.1016/j.ijcard.2011.02.037