Cold stress-induced acclimation in rice is mediated by root-specific aquaporins

Cold acclimation process plays a vital role in the survival of chilling- and freezing-tolerant plants subjected to cold temperature stress. However, it remains elusive whether a cold acclimation process enhances root water uptake (a component of chilling tolerance) in chilling-sensitive crops such a...

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Bibliographic Details
Published in:Plant and cell physiology 2012-08, Vol.53 (8), p.1445-1456
Main Authors: Ahamed, Arifa, Murai-Hatano, Mari, Ishikawa-Sakurai, Junko, Hayashi, Hidehiro, Kawamura, Yukio, Uemura, Matsuo
Format: Article
Language:English
Subjects:
Acclimatization
Aquaporins - genetics
Aquaporins - physiology
Cold Temperature
Cold-Shock Response
Gene Expression Regulation, Plant
Oryza - physiology
Osmosis
Plant Proteins - genetics
Plant Proteins - physiology
Plant Roots - physiology
Plant Shoots - metabolism
Signal Transduction
Water - metabolism
Xylem - physiology
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Summary:Cold acclimation process plays a vital role in the survival of chilling- and freezing-tolerant plants subjected to cold temperature stress. However, it remains elusive whether a cold acclimation process enhances root water uptake (a component of chilling tolerance) in chilling-sensitive crops such as rice. By analyzing the root hydraulic conductivity under cold stress for a prolonged time, we found that cold stress induced a gradual increase in root osmotic hydraulic conductivity [Lp(r(os))]. Compared with the control treatment (roots and shoots at 25°C), low root temperature (LRT) treatment (roots at 10°C; shoots at 25°C) dramatically reduced Lp(r(os)) within 1 h. However, Lp(r(os)) gradually increased during prolonged LRT treatment and it reached 10-fold higher values at day 5. Moreover, a coordinated up-regulation of root aquaporin gene expression, particularly OsPIP2;5, was observed during LRT treatment. Further, comparison of aquaporin gene expression under root-only chilling (LRT) and whole-plant chilling conditions, and in the roots of intact plants vs. shootless plants, suggests that a shoot to root signal is necessary for inducing the expression of aquaporin genes in the root. Collectively, these results demonstrate that a cold acclimation process for root water uptake functions in rice and is possibly regulated through aquaporins.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pcs089