Estradiol protects adipose tissue-derived stem cells against H(2)O(2)-induced toxicity

Oxidative stress is associated with various pathophysiological processes, including cell survival, adhesion, apoptosis, and cancer. In the present study, we aimed to evaluate the effects of H(2)O(2)-induced toxicity on adipose tissue-derived stem cells (ADSCs) and whether 17β-estradiol (E(2)) has pr...

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Bibliographic Details
Published in:Journal of biochemical and molecular toxicology 2012-08, Vol.26 (8), p.301-307
Main Authors: Taskiran, Dilek, Evren, Vedat
Format: Article
Language:English
Subjects:
Adult Stem Cells - drug effects
Adult Stem Cells - physiology
Animals
Cell Differentiation
Cell Shape - drug effects
Cell Survival - drug effects
Cells, Cultured
Chondrogenesis
Estradiol - pharmacology
Estrogens - pharmacology
Hydrogen Peroxide - pharmacology
Intra-Abdominal Fat - cytology
Lipid Peroxidation - drug effects
Osteogenesis
Oxidants - pharmacology
Oxidative Stress
Rats
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Summary:Oxidative stress is associated with various pathophysiological processes, including cell survival, adhesion, apoptosis, and cancer. In the present study, we aimed to evaluate the effects of H(2)O(2)-induced toxicity on adipose tissue-derived stem cells (ADSCs) and whether 17β-estradiol (E(2)) has protective effects on these cells. ADSCs derived from adult Sprague-Dawley rats were pretreated with different doses of E(2) for 24 h and then exposed to 200 µM H(2)O(2) for 4 h. Incubation of ADSCs with H(2)O(2)-decreased cell viability in a concentration-dependent fashion (p < 0.0001), whereas pretreatment of these cells with E(2) significantly reversed toxicity (p < 0.05), inhibited apoptotic changes, and decreased lipid peroxidation (p < 0.0005). Our findings suggest that E(2) protects ADSCs from oxidative-induced cell death, and therefore, it may be used to improve the survival rate and regenerative capacity of stem cells.
ISSN:1099-0461
DOI:10.1002/jbt.21421