The Phosphorylated Sodium Chloride Cotransporter in Urinary Exosomes Is Superior to Prostasin as a Marker for Aldosteronism

Urinary exosomes are vesicles derived from renal tubular epithelial cells. Exosomes often contain several disease-associated proteins and are thus useful targets for identifying biomarkers of disease. Here, we hypothesized that the phosphorylated (active) form of the sodium chloride cotransporter (p...

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Bibliographic Details
Published in:Hypertension (Dallas, Tex. 1979) Tex. 1979), 2012-09, Vol.60 (3), p.741-748
Main Authors: van der Lubbe, Nils, Jansen, Pieter M, Salih, Mahdi, Fenton, Robert A, van den Meiracker, Anton H, Danser, A.H Jan, Zietse, Robert, Hoorn, Ewout J
Format: Article
Language:English
Subjects:
Aldosterone - adverse effects
Aldosterone - pharmacology
Animals
Arterial hypertension. Arterial hypotension
Biological and medical sciences
Biomarkers - urine
Blood and lymphatic vessels
Cardiology. Vascular system
Diet, Sodium-Restricted - adverse effects
Disease Models, Animal
Dose-Response Relationship, Drug
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Exosomes - drug effects
Exosomes - metabolism
Fundamental and applied biological sciences. Psychology
Humans
Hyperaldosteronism - chemically induced
Hyperaldosteronism - diagnosis
Hyperaldosteronism - urine
Kidney Tubules - drug effects
Kidney Tubules - metabolism
Male
Medical sciences
Phosphorylation
Predictive Value of Tests
Rats
Rats, Sprague-Dawley
Sensitivity and Specificity
Serine Endopeptidases - drug effects
Serine Endopeptidases - urine
Sodium Chloride Symporters - drug effects
Sodium Chloride Symporters - urine
Sodium, Dietary - pharmacology
Vertebrates: urinary system
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Summary:Urinary exosomes are vesicles derived from renal tubular epithelial cells. Exosomes often contain several disease-associated proteins and are thus useful targets for identifying biomarkers of disease. Here, we hypothesized that the phosphorylated (active) form of the sodium chloride cotransporter (pNCC) or prostasin could serve as biomarkers for aldosteronism. We tested this in 2 animal models of aldosteronism (aldosterone infusion or low-sodium diet) and in patients with primary aldosteronism. Urinary exosomes were isolated from 24-hour urine or spot urine using ultracentrifugation. In rats, a normal or a high dose of aldosterone for 2, 3, or 8 days increased pNCC 3-fold in urinary exosomes (P
ISSN:0194-911X
1524-4563
DOI:10.1161/HYPERTENSIONAHA.112.198135