Ultraviolet B-induced LGI3 secretion protects human keratinocytes

Leucine‐rich glioma inactivated 3 (LGI3) is known to be expressed mainly in the brain. However, the expression and physiological roles of LGI3 in skin cells remain unknown. In this study, it was found for the first time that LGI3 is expressed mostly by normal human keratinocytes. Furthermore, ELISA...

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Bibliographic Details
Published in:Experimental dermatology 2012-09, Vol.21 (9), p.716-718
Main Authors: Lee, Seung Hoon, Jeong, Yun-Mi, Kim, So-Young, Jeong, Hyo-Soon, Park, Kyoung-Chan, Baek, Kwang Jin, Kwon, Nyoun Soo, Yun, Hye-Young, Kim, Dong-Seok
Format: Article
Language:English
Subjects:
Cell Survival - drug effects
Cells, Cultured
cytokine
Dose-Response Relationship, Radiation
Humans
keratinocytes
Keratinocytes - drug effects
Keratinocytes - radiation effects
Keratinocytes - secretion
LGI3
Phosphorylation - drug effects
Proteins - pharmacology
Proteins - radiation effects
Proteins - secretion
Proto-Oncogene Proteins c-akt - drug effects
Proto-Oncogene Proteins c-akt - metabolism
Proto-Oncogene Proteins c-mdm2 - drug effects
Proto-Oncogene Proteins c-mdm2 - metabolism
Signal Transduction - drug effects
skin
Time Factors
Tumor Suppressor Protein p53 - drug effects
Tumor Suppressor Protein p53 - metabolism
Ultraviolet Rays
UVB
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Summary:Leucine‐rich glioma inactivated 3 (LGI3) is known to be expressed mainly in the brain. However, the expression and physiological roles of LGI3 in skin cells remain unknown. In this study, it was found for the first time that LGI3 is expressed mostly by normal human keratinocytes. Furthermore, ELISA analysis showed that HaCaT human keratinocytes increased LGI3 secretion after exposure to ultraviolet B (UVB) in a time‐ and dose‐dependent manner. We next investigated the possible role of LGI3 in keratinocytes. LGI3 (50 ng/ml) increased survival of HaCaT cells by 20% after UVB irradiation (150 mJ/cm2). It was also found that LGI3 stimulates the phosphorylation of Akt, which is involved in the cell survival‐signalling cascade. Furthermore, LGI3 led to the phosphorylation of MDM2 and subsequent p53 degradation. Taken together, the data suggest that LGI3 may regulate p53 levels and that keratinocyte‐derived LGI3 may act as a novel cytokine for skin homoeostasis.
ISSN:0906-6705
1600-0625
DOI:10.1111/j.1600-0625.2012.01550.x