The effect of smoking on expression of IL-6 and antioxidants in pancreatic fluids and tissues in patients with chronic pancreatitis

Abstract Objectives The present study was conducted to ascertain whether cigarette smoke induces oxidative stress in the human pancreas concurrently with inflammation. Materials and methods The influence of tobacco smoking on the serum level of interleukin-6 (IL-6), on the activities of glutathione...

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Published in:Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] 2012-07, Vol.12 (4), p.295-304
Main Authors: Śliwińska-Mossoń, Mariola, Milnerowicz, Halina, Jabłonowska, Monika, Milnerowicz, Stanisław, Nabzdyk, Stanisław, Rabczyński, Jerzy
Format: Article
Language:English
Subjects:
Adult
Aged
Antioxidants
Antioxidants - analysis
Body Fluids - chemistry
Body Fluids - enzymology
Chronic pancreatitis
Endocrinology & Metabolism
Female
Gastroenterology and Hepatology
Glutathione Peroxidase - metabolism
Humans
Immunohistochemistry
Interleukin-6 - blood
Male
Metallothionein - analysis
Middle Aged
Oxidative Stress
Pancreas - metabolism
Pancreatic fluid
Pancreatitis, Chronic - etiology
Pancreatitis, Chronic - metabolism
Smoking
Smoking - adverse effects
Superoxide Dismutase - metabolism
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Summary:Abstract Objectives The present study was conducted to ascertain whether cigarette smoke induces oxidative stress in the human pancreas concurrently with inflammation. Materials and methods The influence of tobacco smoking on the serum level of interleukin-6 (IL-6), on the activities of glutathione peroxidase (GPx) and copper–zinc superoxide dismutase (Cu/Zn SOD) as well as on the metallothionein (MT) level in the pancreatic pseudocyst fluid and its immunohistochemical localization in tissues of non-smoking ( n  = 9) and smoking ( n  = 12) patients with diagnosed chronic pancreatitis (CP) was measured. The concentration of interleukin-6 and metallothionein was determined by means of ELISA and the radioisotopic method, respectively. The enzyme activities in the fluid were assayed by the colorimetric method. Samples of tissues of normal pancreas ( n  = 4) and CP (non-smoking n  = 7; smoking n  = 12) were verified histopathologically and then IL-6, MT and enzymes were localized by immunohistochemical staining using the monoclonal anti-human antibody. Results The concentrations of metallothionein and interleukin-6 were significantly higher in smoking patients with CP (as compared with the non-smoking population ( p  
ISSN:1424-3903
1424-3911
DOI:10.1016/j.pan.2012.04.007