Anthraquinone derivative O,O'-bis-(3'-iodopropyl)-1,4-dihydroxyanthraquinone modulates immune response and improves experimental autoimmune encephalomyelitis

• Published in: International immunopharmacology 2012-10, Vol.14 (2), p.127-132
• Main Authors: Alves, Caio C S, Castro, Sandra B R, Costa, Cristiane F, Dias, Alyria T, Alves, Chrystian J, Rodrigues, Michele F, Teixeira, Henrique C, Almeida, Mauro V, Ferreira, Ana Paula
• Format: Article
• Language: English
• Subjects: Animals; Cell Line; Cell Movement - drug effects; Cell Survival - drug effects; Cytokines - metabolism; Encephalomyelitis, Autoimmune, Experimental - drug therapy; Encephalomyelitis, Autoimmune, Experimental - immunology; Female; Humans; Immunity - drug effects; Immunomodulation; Inflammation Mediators - metabolism; Macrophages, Peritoneal - drug effects; Macrophages, Peritoneal - pathology; Mice; Mice, Inbred C57BL; Mitoxantrone - analogs & derivatives; Mitoxantrone - therapeutic use; Multiple Sclerosis - drug therapy; Multiple Sclerosis - immunology; Spinal Cord - drug effects; Spinal Cord - metabolism; Spinal Cord - pathology
• ISSN: 1878-1705
• DOI: 10.1016/j.intimp.2012.06.013

The present study investigated the effects of the anthraquinone derivative (O,O'-bis-(3'-iodopropyl)-1,4-dihidroxyanthraquinone - DIPDHAQ), mitoxantrone analog, in an experimental autoimmune encephalomyelitis (EAE) model. The results showed that DIPDHAQ treatment improved the clinical signs of the disease (n=10; vehicle: 3.8 ± 0.3; DIPDHAQ: 1.4 ± 0.9). The improvement was associated with a decrease of inflammatory cells, demyelination, IL-17, IFN-γ, IL-12p40, IL-6, TGF-β, CCL5 and CCL20 levels in the spinal cord. DIPDHAQ presented a low cytotoxicity when in vitro assays were performed. Therefore, the findings suggest a major role for DIPDHAQ in multiple sclerosis, disease characterized as an autoimmune inflammatory disorder against myelin proteins of the brain and spinal cord. The attenuation of inflammation and consequently improvement of clinical signs, involving a decrease of pro-inflammatory cytokines and the low cytotoxicity of DIPDHAQ, suggest that this compound could be used as an alternative treatment for autoimmune diseases in the central nervous system.