Determination of H(N),H (α) and H (N),C' coupling constants in (13)C, (15)N-labeled proteins

Sensitive three-dimensional NMR experiments, based on the E.COSY principle, are presented for the measurement of the (3)J(H(N),H(α)) and (3)J(H(N),C') coupling constants in uniformly (13)C- and (15)N-labeled proteins. They employ gradient coherence selection in combination with the sensitivity...

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Bibliographic Details
Published in:Journal of biomolecular NMR 1994-03, Vol.4 (2), p.231-240
Main Authors: Weisemann, R, Rüterjans, H, Schwalbe, H, Schleucher, J, Bermel, W, Griesinger, C
Format: Article
Language:English
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Summary:Sensitive three-dimensional NMR experiments, based on the E.COSY principle, are presented for the measurement of the (3)J(H(N),H(α)) and (3)J(H(N),C') coupling constants in uniformly (13)C- and (15)N-labeled proteins. They employ gradient coherence selection in combination with the sensitivity enhancement method in HSQC-type spectra (Cavanagh et al., 1991; Palmer et al., 1991). In most cases, the two measured coupling constants unambiguously define the ϕ-angle for protein structure determination. The method is applied to uniformly (13)C, (15)N-labeled ribonuclease T(1).
ISSN:0925-2738
DOI:10.1007/BF00175250