Fabrication of micropillar substrates using replicas of alpha-particle irradiated and chemically etched PADC films

We proposed a simple method to fabricate micropillar substrates. Polyallyldiglycol carbonate (PADC) films were irradiated by alpha particles and then chemically etched to form a cast with micron-scale spherical pores. A polydimethylsiloxane (PDMS) replica of this PADC film gave a micropillar substra...

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Bibliographic Details
Published in:Applied radiation and isotopes 2012-07, Vol.70 (7), p.1432-1435
Main Authors: Ng, C.K.M., Chong, E.Y.W., Roy, V.A.L., Cheung, K.M.C., Yeung, K.W.K., Yu, K.N.
Format: Article
Language:English
Subjects:
Alpha particle
Cell proliferation
HeLa cells
Micropillar substrate
PADC
PDMS
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Summary:We proposed a simple method to fabricate micropillar substrates. Polyallyldiglycol carbonate (PADC) films were irradiated by alpha particles and then chemically etched to form a cast with micron-scale spherical pores. A polydimethylsiloxane (PDMS) replica of this PADC film gave a micropillar substrate with micron-scale spherical pillars. HeLa cells cultured on such a micropillar substrate had significantly larger percentage of cells entering S-phase, attached cell numbers and cell spreading areas. ► We proposed a simple method to fabricate micropillar substrates. ► Polyallyldiglycol carbonate films were irradiated and etched to form casts. ► Polydimethylsiloxane replica then formed the micropillar substrates. ► Attachment and proliferation of HeLa cells were enhanced on these substrates.
ISSN:0969-8043
1872-9800
DOI:10.1016/j.apradiso.2011.11.019