Exploration of the binding proteins of perfluorooctane sulfonate by a T7 phage display screen

Perfluorooctane sulfonate (PFOS) is a pollutant widely found throughout nature and is toxic to animals. We created a PFOS analogue on a polyethylene glycol polyacrylamide copolymer and isolated peptides that preferentially bound the PFOS analogue using a T7 phage display system. Bioinformatic analys...

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Bibliographic Details
Published in:Bioorganic & medicinal chemistry 2012-07, Vol.20 (13), p.3985-3990
Main Authors: Miyano, Yuka, Tsukuda, Senko, Sakimoto, Ippei, Takeuchi, Ryo, Shimura, Satomi, Takahashi, Noriyuki, Kusayanagi, Tomoe, Takakusagi, Yoichi, Okado, Mami, Matsumoto, Yuki, Takakusagi, Kaori, Takeuchi, Toshifumi, Kamisuki, Shinji, Nakazaki, Atsuo, Ohta, Keisuke, Miura, Masahiko, Kuramochi, Kouji, Mizushina, Yoshiyuki, Kobayashi, Susumu, Sugawara, Fumio, Sakaguchi, Kengo
Format: Article
Language:English
Subjects:
Alkanesulfonic Acids - chemistry
Alkanesulfonic Acids - metabolism
Amino Acid Sequence
Animals
Bioinformatics
Biological and medical sciences
Biosensors
Carrier Proteins - metabolism
CD14
CD14 antigen
Cell Line
Computational Biology
Computer programs
Copolymers
Differentiation
Fluorocarbons - chemistry
Fluorocarbons - metabolism
Humans
Lipopolysaccharide Receptors - genetics
Lipopolysaccharide Receptors - metabolism
Macrophages
Medical sciences
Mice
Monocytes
Peptide Library
Perfluorooctane sulfonate
Phage display
Pharmacology. Drug treatments
Pollutants
Polyethylene glycol
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
RELIC
Software
Solid-phase synthesis
Surface Plasmon Resonance
Tumor Necrosis Factor-alpha - metabolism
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Summary:Perfluorooctane sulfonate (PFOS) is a pollutant widely found throughout nature and is toxic to animals. We created a PFOS analogue on a polyethylene glycol polyacrylamide copolymer and isolated peptides that preferentially bound the PFOS analogue using a T7 phage display system. Bioinformatic analysis using the FASTAskan program on the RELIC bioinformatics server showed several human proteins that likely bound PFOS. Among them, we confirmed binding between PFOS and a recombinant soluble form of monocyte differentiation antigen CD14 (sCD14) by a surface plasmon biosensor. Furthermore, PFOS inhibited TNF-α production induced by the sCD14 in mouse macrophage RAW264.7 cells.
ISSN:0968-0896
1464-3391
DOI:10.1016/j.bmc.2012.05.016