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Exploration of the binding proteins of perfluorooctane sulfonate by a T7 phage display screen
Perfluorooctane sulfonate (PFOS) is a pollutant widely found throughout nature and is toxic to animals. We created a PFOS analogue on a polyethylene glycol polyacrylamide copolymer and isolated peptides that preferentially bound the PFOS analogue using a T7 phage display system. Bioinformatic analys...
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| Published in: | Bioorganic & medicinal chemistry 2012-07, Vol.20 (13), p.3985-3990 |
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| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c482t-83df02e6955e0ca47bfa672bc4270e0538002816221c2a2710e641fa315bf8b83 |
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| cites | cdi_FETCH-LOGICAL-c482t-83df02e6955e0ca47bfa672bc4270e0538002816221c2a2710e641fa315bf8b83 |
| container_end_page | 3990 |
| container_issue | 13 |
| container_start_page | 3985 |
| container_title | Bioorganic & medicinal chemistry |
| container_volume | 20 |
| creator | Miyano, Yuka Tsukuda, Senko Sakimoto, Ippei Takeuchi, Ryo Shimura, Satomi Takahashi, Noriyuki Kusayanagi, Tomoe Takakusagi, Yoichi Okado, Mami Matsumoto, Yuki Takakusagi, Kaori Takeuchi, Toshifumi Kamisuki, Shinji Nakazaki, Atsuo Ohta, Keisuke Miura, Masahiko Kuramochi, Kouji Mizushina, Yoshiyuki Kobayashi, Susumu Sugawara, Fumio Sakaguchi, Kengo |
| description | Perfluorooctane sulfonate (PFOS) is a pollutant widely found throughout nature and is toxic to animals. We created a PFOS analogue on a polyethylene glycol polyacrylamide copolymer and isolated peptides that preferentially bound the PFOS analogue using a T7 phage display system. Bioinformatic analysis using the FASTAskan program on the RELIC bioinformatics server showed several human proteins that likely bound PFOS. Among them, we confirmed binding between PFOS and a recombinant soluble form of monocyte differentiation antigen CD14 (sCD14) by a surface plasmon biosensor. Furthermore, PFOS inhibited TNF-α production induced by the sCD14 in mouse macrophage RAW264.7 cells. |
| doi_str_mv | 10.1016/j.bmc.2012.05.016 |
| format | article |
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We created a PFOS analogue on a polyethylene glycol polyacrylamide copolymer and isolated peptides that preferentially bound the PFOS analogue using a T7 phage display system. Bioinformatic analysis using the FASTAskan program on the RELIC bioinformatics server showed several human proteins that likely bound PFOS. Among them, we confirmed binding between PFOS and a recombinant soluble form of monocyte differentiation antigen CD14 (sCD14) by a surface plasmon biosensor. 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Drug treatments ; Pollutants ; Polyethylene glycol ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; RELIC ; Software ; Solid-phase synthesis ; Surface Plasmon Resonance ; Tumor Necrosis Factor-alpha - metabolism</subject><ispartof>Bioorganic & medicinal chemistry, 2012-07, Vol.20 (13), p.3985-3990</ispartof><rights>2012 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-83df02e6955e0ca47bfa672bc4270e0538002816221c2a2710e641fa315bf8b83</citedby><cites>FETCH-LOGICAL-c482t-83df02e6955e0ca47bfa672bc4270e0538002816221c2a2710e641fa315bf8b83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26079225$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22658539$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miyano, Yuka</creatorcontrib><creatorcontrib>Tsukuda, Senko</creatorcontrib><creatorcontrib>Sakimoto, Ippei</creatorcontrib><creatorcontrib>Takeuchi, Ryo</creatorcontrib><creatorcontrib>Shimura, Satomi</creatorcontrib><creatorcontrib>Takahashi, Noriyuki</creatorcontrib><creatorcontrib>Kusayanagi, Tomoe</creatorcontrib><creatorcontrib>Takakusagi, Yoichi</creatorcontrib><creatorcontrib>Okado, Mami</creatorcontrib><creatorcontrib>Matsumoto, Yuki</creatorcontrib><creatorcontrib>Takakusagi, Kaori</creatorcontrib><creatorcontrib>Takeuchi, Toshifumi</creatorcontrib><creatorcontrib>Kamisuki, Shinji</creatorcontrib><creatorcontrib>Nakazaki, Atsuo</creatorcontrib><creatorcontrib>Ohta, Keisuke</creatorcontrib><creatorcontrib>Miura, Masahiko</creatorcontrib><creatorcontrib>Kuramochi, Kouji</creatorcontrib><creatorcontrib>Mizushina, Yoshiyuki</creatorcontrib><creatorcontrib>Kobayashi, Susumu</creatorcontrib><creatorcontrib>Sugawara, Fumio</creatorcontrib><creatorcontrib>Sakaguchi, Kengo</creatorcontrib><title>Exploration of the binding proteins of perfluorooctane sulfonate by a T7 phage display screen</title><title>Bioorganic & medicinal chemistry</title><addtitle>Bioorg Med Chem</addtitle><description>Perfluorooctane sulfonate (PFOS) is a pollutant widely found throughout nature and is toxic to animals. We created a PFOS analogue on a polyethylene glycol polyacrylamide copolymer and isolated peptides that preferentially bound the PFOS analogue using a T7 phage display system. Bioinformatic analysis using the FASTAskan program on the RELIC bioinformatics server showed several human proteins that likely bound PFOS. Among them, we confirmed binding between PFOS and a recombinant soluble form of monocyte differentiation antigen CD14 (sCD14) by a surface plasmon biosensor. Furthermore, PFOS inhibited TNF-α production induced by the sCD14 in mouse macrophage RAW264.7 cells.</description><subject>Alkanesulfonic Acids - chemistry</subject><subject>Alkanesulfonic Acids - metabolism</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Carrier Proteins - metabolism</subject><subject>CD14</subject><subject>CD14 antigen</subject><subject>Cell Line</subject><subject>Computational Biology</subject><subject>Computer programs</subject><subject>Copolymers</subject><subject>Differentiation</subject><subject>Fluorocarbons - chemistry</subject><subject>Fluorocarbons - metabolism</subject><subject>Humans</subject><subject>Lipopolysaccharide Receptors - genetics</subject><subject>Lipopolysaccharide Receptors - metabolism</subject><subject>Macrophages</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Monocytes</subject><subject>Peptide Library</subject><subject>Perfluorooctane sulfonate</subject><subject>Phage display</subject><subject>Pharmacology. Drug treatments</subject><subject>Pollutants</subject><subject>Polyethylene glycol</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>RELIC</subject><subject>Software</subject><subject>Solid-phase synthesis</subject><subject>Surface Plasmon Resonance</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><issn>0968-0896</issn><issn>1464-3391</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp9kEFLHDEYhoO06Gr7A3opuRS8zDTJJJkMnorYKghe9Cghk_miWWaTaTIj7r83y27tzdMHL8_78fIg9I2SmhIqf67rfmNrRiiriahLcoRWlEteNU1HP6EV6aSqiOrkCTrNeU0IYbyjx-iEMSmUaLoVerx6ncaYzOxjwNHh-Rlw78PgwxOeUpzBh7zLJ0huXGKK0c4mAM7L6GIwc6G32OD7Fk_P5gnw4PM0mi3ONgGEL-izM2OGr4d7hh5-X91fXle3d39uLn_dVpYrNleqGRxhIDshgFjD294Z2bLectYSIKJRZbmikjFqmWEtJSA5daahoneqV80ZOt__LZP_LpBnvfHZwjiWqXHJmpKGK8o7tkPpHrUp5pzA6Sn5jUnbAumdVb3WxareWdVE6JKUzvfD-6XfwPDe-KexAD8OgMnWjC6ZYH3-z0nSdoyJwl3sOSgyXjwkna2HYGHwCeysh-g_mPEGNtqUGQ</recordid><startdate>20120701</startdate><enddate>20120701</enddate><creator>Miyano, Yuka</creator><creator>Tsukuda, Senko</creator><creator>Sakimoto, Ippei</creator><creator>Takeuchi, Ryo</creator><creator>Shimura, Satomi</creator><creator>Takahashi, Noriyuki</creator><creator>Kusayanagi, Tomoe</creator><creator>Takakusagi, Yoichi</creator><creator>Okado, Mami</creator><creator>Matsumoto, Yuki</creator><creator>Takakusagi, Kaori</creator><creator>Takeuchi, Toshifumi</creator><creator>Kamisuki, Shinji</creator><creator>Nakazaki, Atsuo</creator><creator>Ohta, Keisuke</creator><creator>Miura, Masahiko</creator><creator>Kuramochi, Kouji</creator><creator>Mizushina, Yoshiyuki</creator><creator>Kobayashi, Susumu</creator><creator>Sugawara, Fumio</creator><creator>Sakaguchi, Kengo</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20120701</creationdate><title>Exploration of the binding proteins of perfluorooctane sulfonate by a T7 phage display screen</title><author>Miyano, Yuka ; Tsukuda, Senko ; Sakimoto, Ippei ; Takeuchi, Ryo ; Shimura, Satomi ; Takahashi, Noriyuki ; Kusayanagi, Tomoe ; Takakusagi, Yoichi ; Okado, Mami ; Matsumoto, Yuki ; Takakusagi, Kaori ; Takeuchi, Toshifumi ; Kamisuki, Shinji ; Nakazaki, Atsuo ; Ohta, Keisuke ; Miura, Masahiko ; Kuramochi, Kouji ; Mizushina, Yoshiyuki ; Kobayashi, Susumu ; Sugawara, Fumio ; Sakaguchi, Kengo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-83df02e6955e0ca47bfa672bc4270e0538002816221c2a2710e641fa315bf8b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Alkanesulfonic Acids - chemistry</topic><topic>Alkanesulfonic Acids - metabolism</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>Biosensors</topic><topic>Carrier Proteins - metabolism</topic><topic>CD14</topic><topic>CD14 antigen</topic><topic>Cell Line</topic><topic>Computational Biology</topic><topic>Computer programs</topic><topic>Copolymers</topic><topic>Differentiation</topic><topic>Fluorocarbons - chemistry</topic><topic>Fluorocarbons - metabolism</topic><topic>Humans</topic><topic>Lipopolysaccharide Receptors - genetics</topic><topic>Lipopolysaccharide Receptors - metabolism</topic><topic>Macrophages</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Monocytes</topic><topic>Peptide Library</topic><topic>Perfluorooctane sulfonate</topic><topic>Phage display</topic><topic>Pharmacology. 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| fulltext | fulltext |
| identifier | ISSN: 0968-0896 |
| ispartof | Bioorganic & medicinal chemistry, 2012-07, Vol.20 (13), p.3985-3990 |
| issn | 0968-0896 1464-3391 |
| language | eng |
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| source | ScienceDirect Journals |
| subjects | Alkanesulfonic Acids - chemistry Alkanesulfonic Acids - metabolism Amino Acid Sequence Animals Bioinformatics Biological and medical sciences Biosensors Carrier Proteins - metabolism CD14 CD14 antigen Cell Line Computational Biology Computer programs Copolymers Differentiation Fluorocarbons - chemistry Fluorocarbons - metabolism Humans Lipopolysaccharide Receptors - genetics Lipopolysaccharide Receptors - metabolism Macrophages Medical sciences Mice Monocytes Peptide Library Perfluorooctane sulfonate Phage display Pharmacology. Drug treatments Pollutants Polyethylene glycol Recombinant Proteins - genetics Recombinant Proteins - metabolism RELIC Software Solid-phase synthesis Surface Plasmon Resonance Tumor Necrosis Factor-alpha - metabolism |
| title | Exploration of the binding proteins of perfluorooctane sulfonate by a T7 phage display screen |
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