Enhancement of the immunogenicity of an alphavirus replicon-based DNA vaccine against classical swine fever by electroporation and coinjection with a plasmid expressing porcine interleukin 2

Highlights ► Electroporation (EP) and IL-2 were tested to improve the immunogenicity of alphavirus-replicon-based vaccine against CSF. ► EP combined with IL-2 resulted in marked enhancement of specific antibody response of the vaccine. ► Specific lymphocyte proliferation, IFN-γ and IL-4 responses we...

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Bibliographic Details
Published in:Vaccine 2012-05, Vol.30 (24), p.3587-3594
Main Authors: Tian, Da-Yong, Sun, Yuan, Wai, Sing Fai, Lee, Fuk Ki, Meng, Qi-Lin, Suen, Kar Man, Wang, Nan, Han, Wen, Li, Su, Li, Yong-Feng, Li, Dan, Ling, Li-Jun, Liao, Ya-Jin, Qiu, Hua-Ji
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic - administration & dosage
Adjuvants, Immunologic - genetics
Allergy and Immunology
Alphavirus
Alphavirus - genetics
Alphavirus replicon
Animals
Antibodies
Antibodies, Viral - blood
Antigens
Cell Proliferation
Classical swine fever
Classical Swine Fever - immunology
Classical Swine Fever - prevention & control
Colony-stimulating factor
Deoxyribonucleic acid
DNA
DNA vaccines
Electroporation
Electroporation - methods
Fever
Gene expression
Genetic Vectors
Hog cholera
Hogs
Immune response
Immunity
Immunization
Immunogenicity
interferon-gamma
Interferon-gamma - secretion
Interleukin 2
Interleukin 4
Interleukin-2 - administration & dosage
Interleukin-2 - genetics
Interleukin-4 - secretion
intramuscular injection
Lymphatic system
lymphocyte proliferation
Lymphocytes
Lymphocytes - immunology
Plasmids
Plasmids - administration & dosage
recombinant vaccines
sterilizing
Swine
Vaccines
Vaccines, DNA - administration & dosage
Vaccines, DNA - genetics
Vaccines, DNA - immunology
Vaccines, Synthetic - administration & dosage
Vaccines, Synthetic - genetics
Vaccines, Synthetic - immunology
viral load
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Summary:Highlights ► Electroporation (EP) and IL-2 were tested to improve the immunogenicity of alphavirus-replicon-based vaccine against CSF. ► EP combined with IL-2 resulted in marked enhancement of specific antibody response of the vaccine. ► Specific lymphocyte proliferation, IFN-γ and IL-4 responses were also increased significantly. ► Pigs immunized with the vaccine together with IL-2 by EP were protected completely from lethal challenge.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2012.03.049