Development and characterization of 21 polymorphic microsatellite loci in the aphidophagous gall midge, Aphidoletes aphidimyza (Diptera: Cecidomyiidae)

We have developed and characterized 21 microsatellite markers in the aphidophagous gall midge Aphidoletes aphidimyza (Rondani) (Diptera: Cecidomyiidae). All 21 loci tested were polymorphic: the number of alleles ranged from 2 to 17. Allelic richness and observed heterozygosities were higher in femal...

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Bibliographic Details
Published in:Applied entomology and zoology 2012-05, Vol.47 (2), p.165-171
Main Authors: Hinomoto, Norihide, Higaki, Tomomi, Abe, Junichiro, Yamane, Masafumi, Yano, Eizi
Format: Article
Language:English
Subjects:
Agriculture
Aphidoletes aphidimyza
Applied Ecology
Biomedical and Life Sciences
Cecidomyiidae
Diptera
Entomology
Environmental Management
Gene polymorphism
Genetic markers
Genetic structure
Heterozygosity
Life Sciences
Microsatellites
Plant Pathology
Sex chromosomes
Sex differences
Technical Note
Zoology
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Summary:We have developed and characterized 21 microsatellite markers in the aphidophagous gall midge Aphidoletes aphidimyza (Rondani) (Diptera: Cecidomyiidae). All 21 loci tested were polymorphic: the number of alleles ranged from 2 to 17. Allelic richness and observed heterozygosities were higher in females than in males. Several loci had no heterozygosity in males, suggesting that the loci were located on sex chromosomes or E-chromosomes, common to cecidomyiids. The high polymorphism detected in this study suggests the markers will be of value in analyzing genetic structure of field populations.
ISSN:0003-6862
1347-605X
DOI:10.1007/s13355-012-0104-z