Conservation of genomic imprinting at the NDN, MAGEL2 and MEST loci in pigs

Imprinted genes have important effects on the regulation of fetal growth, development, and postnatal behavior. However, the study of imprinted genes has been limited in mammalian species other than human and mouse. Therefore, the study of porcine imprinted genes is useful for defining the extent of...

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Bibliographic Details
Published in:Genes & Genetic Systems 2012, Vol.87(1), pp.53-58
Main Authors: Zhang, Feng Wei, Han, Zheng Bin, Deng, Chang Yan, He, Hong Juan, Wu, Qiong
Format: Article
Language:English
Subjects:
Animals
Antigens, Neoplasm - genetics
Antigens, Neoplasm - metabolism
Base Sequence
Cardiac muscle
Conservation
Discrimination
Female
Fetuses
Gene Expression Profiling
Genomic Imprinting
Hybridization, Genetic
Imprinting
Kidney
Liver
Lung
MAGEL2
Male
MEST
Molecular Sequence Data
Muscles
NDN
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Organ Specificity
Ovaries
pig
Pituitary
Polymorphism, Single Nucleotide
Proteins - genetics
Proteins - metabolism
Real-Time Polymerase Chain Reaction
Sequence Analysis, DNA
Single-nucleotide polymorphism
Skeletal muscle
Small intestine
Spleen
Stomach
Sus scrofa - genetics
Testes
Transcription, Genetic
Uterus
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Summary:Imprinted genes have important effects on the regulation of fetal growth, development, and postnatal behavior. However, the study of imprinted genes has been limited in mammalian species other than human and mouse. Therefore, the study of porcine imprinted genes is useful for defining the extent of conservation of genomic imprinting among different species. In this study, the imprinting status of porcine NDN, MAGEL2 and MEST genes was determined by direct sequencing of the cDNAs and detection of single nucleotide polymorphisms (SNPs) identified in individuals from reciprocal crosses between Meishan and Large White pigs for allele discrimination. The analysis was carried out in 13 different tissues (skeletal muscle, fat, pituitary gland, heart, lung, liver, kidney, spleen, stomach, small intestine, uterus, ovary and testis) from 12 two-month-old piglets. Imprinting analysis showed that NDN and MAGEL2 were paternally expressed in all tissues where the genes were expressed as in human and mouse. Interestingly, MEST showed tissue-specific imprinting, being paternally expressed in skeletal muscle, fat, pituitary gland, heart, kidney, lung, stomach and uterus, and maternally expressed in spleen and liver.
ISSN:1341-7568
1880-5779
DOI:10.1266/ggs.87.53