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Genetic Map of AFLP Markers in the Rat (Rattus norvegicus) Derived from the H × B/Ipcv and B × H/Cub Sets of Recombinant Inbred Strains

The amplified fragment length polymorphism (AFLP) technique has been used to enhance marker density in a large set of recombinant inbred strains (H × B and B × H) derived from a spontaneously hypertensive rat (SHR/OlaIpcv) and a Brown-Norway (BN.lx/Cub) inbred strain. Thirteen different primer combi...

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Published in:Biochemical genetics 2003-04, Vol.41 (3-4), p.77-89
Main Authors: Bonné, Anita C, M, Den Bieman, Maria G, Gillissen, Gert F, Kren, Vladimír, Krenová, Drahomíra, Bílá, Vlasta, Zídek, Václav, Kostka, Vlastimil, Musilová, Alena, Pravenec, Michal, van Zutphen, Bert F, van Lith, Hein A
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container_issue 3-4
container_start_page 77
container_title Biochemical genetics
container_volume 41
creator Bonné, Anita C
M
Den Bieman, Maria G
Gillissen, Gert F
Kren, Vladimír
Krenová, Drahomíra
Bílá, Vlasta
Zídek, Václav
Kostka, Vlastimil
Musilová, Alena
Pravenec, Michal
van Zutphen, Bert F
M
van Lith, Hein A
description The amplified fragment length polymorphism (AFLP) technique has been used to enhance marker density in a large set of recombinant inbred strains (H × B and B × H) derived from a spontaneously hypertensive rat (SHR/OlaIpcv) and a Brown-Norway (BN.lx/Cub) inbred strain. Thirteen different primer combinations were tested and a total of 191 polymorphic bands were detected. From these polymorphic bands 89 AFLP markers could be assigned to specific chromosomes. Several of these AFLP markers were mapped to regions with low marker density, thus filling up gaps in the existing genetic map of these recombinant inbred strains. These results substantiate the value of the AFLP technology in increasing marker density in genetic maps.[PUBLICATION ABSTRACT]
doi_str_mv 10.1023/A:1022069816135
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subjects Amplified fragment length polymorphism
Chromosomes
Gene mapping
Genetic diversity
Inbreeding
Primers
Rattus norvegicus
title Genetic Map of AFLP Markers in the Rat (Rattus norvegicus) Derived from the H × B/Ipcv and B × H/Cub Sets of Recombinant Inbred Strains
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