The relationship between caspase-3 expression and cell proliferation in rat lung squamous cell carcinoma

• Published in: Zhongguo fei ai za zhi 2002-12, Vol.5 (6), p.440-443
• Main Authors: Chen, Honglei, Diao, Luming, Chen, Fuchun, Liu, Xuan, Zhang, Yuxia, Ye, Bo, Liu, Mingqiu
• Format: Article
• Language: Chinese
• ISSN: 1009-3419
• DOI: 10.3779/j.issn.1009-3419.2002.06.13

BACKGROUNDTo analyse the relationship between caspase-3 expression and cell proliferation, and to find molecular-biology markers to adjust canceration during rat lung squamous cell carcinogenesis.METHODSThe 3-methylcholanthrene(MCA) and diethyinitrosamine (DEN) were used to induce lung squamous cell carcinoma by intra-left lobar-bronchial instillation in 50 Wistar rats, and 10 normal rats as controls. Expression of caspase-3 and PCNA were evaluated by immunohistochemistry (IHC).RESULTSCaspase-3 protein positive rate was 44.12% in 34 rat lung squamous cell carcinomas, and positive coefficient value was 1.38±0.95, which were significantly lower than that of normal bronchial epithelium (P=0.007, P < 0.01) and premalignant lesions (P < 0.05, P < 0.05). The mean PCNA-labeling indexes (PCNA-LI) of normal rat bronchial epithelium, premalignant lesions and lung cancer were 14.10±5.02, 28.13±8.72 and 41.88±14.24 (P < 0.05), respectively. There was a negative correlation between caspase 3 and PCNA LI (r=-0.730 6, P < 0