Ghrelin stimulates myogenic differentiation in a mouse muscle satellite cell line and in primary cultures of bovine myoblasts

Ghrelin is an acylated hormone that influences food intake, energy metabolism and reproduction, among others. Ghrelin may also stimulate proliferating myoblast cell differentiation and multinucleated myotube fusion. The aim of this work was to assess the effect of human ghrelin (hGHRL) and human ghr...

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Published in:Journal of animal physiology and animal nutrition 2012-08, Vol.96 (4), p.725-738
Main Authors: Montoya‐Flores, D, Mora, O, Tamariz, E, González‐Dávalos, L, González‐Gallardo, A, Antaramian, A, Shimada, A, Varela‐Echavarría, A, Romano‐Muñoz, J. L
Format: Article
Language:English
Subjects:
Animals
bovines
Cattle
cell differentiation
Cell Differentiation - drug effects
Cell Line
Cells
energy metabolism
food intake
gene expression
Gene Expression Regulation - drug effects
ghrelin
Ghrelin - pharmacology
i28 cell line
livestock production
messenger RNA
Mice
muscle development
myoblast
myoblasts
Myoblasts - cytology
Myoblasts - drug effects
Myoblasts - physiology
Myogenin - genetics
Myogenin - metabolism
PAX7 Transcription Factor - genetics
PAX7 Transcription Factor - metabolism
protein synthesis
Proteins
Rodents
Satellite Cells, Skeletal Muscle - cytology
Satellite Cells, Skeletal Muscle - drug effects
Satellite Cells, Skeletal Muscle - physiology
Satellites
skeletal muscle
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Summary:Ghrelin is an acylated hormone that influences food intake, energy metabolism and reproduction, among others. Ghrelin may also stimulate proliferating myoblast cell differentiation and multinucleated myotube fusion. The aim of this work was to assess the effect of human ghrelin (hGHRL) and human ghrelin fragment 1–18 (hGHRL1–18) on myoblast differentiation by means of mRNA expression and protein level. Two types of cells were tested, the cell line i28 obtained from mouse skeletal muscle and primary cultures of bovine myoblasts. Both ghrelin and its N‐terminal fragment hGHRL1–18 were used at concentrations of 0, 0.01, 0.1, 1, 10 and 100 nm. Treatments were applied to pre‐confluent cultures and were maintained for 4 days. We determined that between 0.1 and 100 nm, hGHRL and hGRHL1–18 had similar effects on myogenic differentiation of i28 cells (p 
ISSN:0931-2439
1439-0396
DOI:10.1111/j.1439-0396.2011.01201.x