Validation of a microscale extraction and high-throughput UHPLC-QTOF-MS analysis method for huperzine A in Huperzia

ABSTRACT Herein we report on an improved method for the microscale extraction of huperzine A (HupA), an acetylcholinesterase‐inhibiting alkaloid, from as little as 3 mg of tissue homogenate from the clubmoss Huperzia squarrosa (G. Forst.) Trevis with 99.95% recovery. We also validated a novel UHPLC‐...

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Bibliographic Details
Published in:Biomedical chromatography 2012-10, Vol.26 (10), p.1191-1195
Main Authors: Cuthbertson, Daniel, Piljac-Žegarac, Jasenka, Lange, Bernd Markus
Format: Article
Language:English
Subjects:
alkaloid
Alkaloids - analysis
Chromatography, High Pressure Liquid - methods
High-Throughput Screening Assays
Huperzia
Huperzia - chemistry
huperzine A
Limit of Detection
Linear Models
mass spectrometry
microscale extraction
Plant Extracts - chemistry
Reproducibility of Results
Sesquiterpenes - analysis
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Summary:ABSTRACT Herein we report on an improved method for the microscale extraction of huperzine A (HupA), an acetylcholinesterase‐inhibiting alkaloid, from as little as 3 mg of tissue homogenate from the clubmoss Huperzia squarrosa (G. Forst.) Trevis with 99.95% recovery. We also validated a novel UHPLC‐QTOF‐MS method for the high‐throughput analysis of H. squarrosa extracts in only 6 min, which, in combination with the very low limit of detection (20 pg on column) and the wide linear range for quantification (20–10,000 pg on column), allow for a highly efficient screening of extracts containing varying amounts of HupA. Utilization of this methodology has the potential to conserve valuable plant resources. Copyright © 2012 John Wiley & Sons, Ltd.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.2677