The effects of n-3 PUFA and intestinal lymph drainage on high-mobility group box 1 and Toll-like receptor 4 mRNA in rats with intestinal ischaemia–reperfusion injury

The aim of the present study was to investigate the impacts of n-3 PUFA and lymph drainage (D) on intestinal ischaemia–reperfusion (I/R) injury in rats. A total of forty-eight Sprague–Dawley male rats were randomly divided into three groups (n 16): normal diet (N), enteral nutrition (EN) and EN plus...

Full description

Saved in:
Bibliographic Details
Published in:British journal of nutrition 2012-09, Vol.108 (5), p.883-892
Main Authors: He, Gui-Zhen, Zhou, Kai-Guo, Zhang, Rui, Chen, Xue-Feng
Format: Article
Language:English
Subjects:
Alanine Transaminase - blood
Animals
Base Sequence
Biological and medical sciences
Cytokines - blood
DNA Probes
Drainage
Endotoxins
Enteral nutrition
Fatty acids
Fatty Acids, Omega-3 - pharmacology
Feeding. Feeding behavior
Fundamental and applied biological sciences. Psychology
HMGB1 Protein - genetics
Immunohistochemistry
Immunology
Intestines - blood supply
Intestines - drug effects
Intestines - metabolism
Ischemia
Large intestine
Liver - metabolism
Lymph
Lymphatic system
Male
NF-kappa B - metabolism
Nitric Oxide - metabolism
Nitric Oxide Synthase - metabolism
Nutrition
Nutritional Immunology
Peroxidase - metabolism
Rats
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
Rodents
Toll-Like Receptor 4 - genetics
Vertebrates: anatomy and physiology, studies on body, several organs or systems
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of the present study was to investigate the impacts of n-3 PUFA and lymph drainage (D) on intestinal ischaemia–reperfusion (I/R) injury in rats. A total of forty-eight Sprague–Dawley male rats were randomly divided into three groups (n 16): normal diet (N), enteral nutrition (EN) and EN plus n-3 PUFA. Each group was further divided into lymph drainage (I/R+D) and non-drainage (I/R) sub-groups (n 8). After 5 d with different nutrition regimens, the rats were subjected to 60 min ischaemia by clamping the superior mesenteric artery, followed by 120 min reperfusion. At the same time, the rats in the I/R+D sub-groups were treated with intestinal lymph drainage for 180 min. Organs were harvested and we detected the cytokine, endotoxin, and expression of Toll-like receptor (TLR) 4 mRNA and its endogenous ligand high-mobility group box 1 (HMGB1). We found that the serum levels of HMGB1, inflammatory cytokine and endotoxin in the three I/R+D sub-groups were significantly lower than those in the N (I/R) and EN (I/R) sub-groups (P 
ISSN:0007-1145
1475-2662
DOI:10.1017/S0007114511006040