"Click & Seed" Approach to the Biomimetic Modification of Material Surfaces

A simple, versatile, protein‐repulsive, substrate‐independent biomimetic surface modification is presented that is based on the creation of a PEO brush on a polydopamine anchoring layer and its capacity for selective follow‐up modifications with various ligands using a copper‐catalyzed alkyne‐azide...

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Bibliographic Details
Published in:Macromolecular bioscience 2012-09, Vol.12 (9), p.1232-1242
Main Authors: Proks, Vladimír, Jaroš, Josef, Pop-Georgievski, Ognen, Kučka, Jan, Popelka, Štěpán, Dvořák, Petr, Hampl, Aleš, Rypáček, František
Format: Article
Language:English
Subjects:
Applied sciences
biomimetic modifications
Biomimetics
Cells, Cultured
Chemical modifications
Chemical reactions and properties
click chemistry
Cyclization
Exact sciences and technology
Humans
Organic polymers
peptide radiolabeling
Physicochemistry of polymers
poly(ethylene oxide)
polydopamine
Surface Properties
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Summary:A simple, versatile, protein‐repulsive, substrate‐independent biomimetic surface modification is presented that is based on the creation of a PEO brush on a polydopamine anchoring layer and its capacity for selective follow‐up modifications with various ligands using a copper‐catalyzed alkyne‐azide cycloaddition reaction. The desired surface concentration of peptide biomimetic ligands can be controlled by adjusting the peptide concentration in the reaction mixture, then measuring the activity of 125I‐radiolabeled peptides that are immobilized on the substrates. The performance of the prepared substrates is tested in cell cultures with MEF cells and a human ECC line. A simple, versatile, protein‐repulsive, substrate‐independent biomimetic surface modification is presented that is based on the creation of a PEO brush on a polydopamine anchoring layer and its capacity for selective follow‐up modifications with various ligands using a copper‐catalyzed alkyne‐azide cycloaddition reaction. The modified surface allows direct cell seeding and controlled cultivation.
ISSN:1616-5187
1616-5195
DOI:10.1002/mabi.201200095