"Click & Seed" Approach to the Biomimetic Modification of Material Surfaces

• Published in: Macromolecular bioscience 2012-09, Vol.12 (9), p.1232-1242
• Main Authors: Proks, Vladimír, Jaroš, Josef, Pop-Georgievski, Ognen, Kučka, Jan, Popelka, Štěpán, Dvořák, Petr, Hampl, Aleš, Rypáček, František
• Format: Article
• Language: English
• Subjects: Applied sciences; biomimetic modifications; Biomimetics; Cells, Cultured; Chemical modifications; Chemical reactions and properties; click chemistry; Cyclization; Exact sciences and technology; Humans; Organic polymers; peptide radiolabeling; Physicochemistry of polymers; poly(ethylene oxide); polydopamine; Surface Properties
• ISSN: 1616-5187; 1616-5195
• DOI: 10.1002/mabi.201200095

A simple, versatile, protein‐repulsive, substrate‐independent biomimetic surface modification is presented that is based on the creation of a PEO brush on a polydopamine anchoring layer and its capacity for selective follow‐up modifications with various ligands using a copper‐catalyzed alkyne‐azide cycloaddition reaction. The desired surface concentration of peptide biomimetic ligands can be controlled by adjusting the peptide concentration in the reaction mixture, then measuring the activity of 125I‐radiolabeled peptides that are immobilized on the substrates. The performance of the prepared substrates is tested in cell cultures with MEF cells and a human ECC line. A simple, versatile, protein‐repulsive, substrate‐independent biomimetic surface modification is presented that is based on the creation of a PEO brush on a polydopamine anchoring layer and its capacity for selective follow‐up modifications with various ligands using a copper‐catalyzed alkyne‐azide cycloaddition reaction. The modified surface allows direct cell seeding and controlled cultivation.