Label-free detection of proteins from dried-suspended droplets using surface enhanced Raman scattering

A simple sample preparation method to obtain rich and reproducible surface-enhanced Raman scattering (SERS) spectra from proteins regardless of their surface properties and dimensions for label-free detection and identification is reported. The method uses colloidal silver nanoparticles (AgNPs) as s...

Full description

Saved in:
Bibliographic Details
Published in:Analyst (London) 2012-06, Vol.137 (11), p.2651-2657
Main Authors: KESKIN, Sercan, CULHA, Mustafa
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Droplets
Drying
Exact sciences and technology
Hydrophobic and Hydrophilic Interactions
Metal Nanoparticles - chemistry
Metal Nanoparticles - ultrastructure
Nanoparticles
Proteins
Proteins - analysis
Raman scattering
Silver - chemistry
Spectra
Spectrometric and optical methods
Spectrum Analysis, Raman
Surface Properties
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A simple sample preparation method to obtain rich and reproducible surface-enhanced Raman scattering (SERS) spectra from proteins regardless of their surface properties and dimensions for label-free detection and identification is reported. The method uses colloidal silver nanoparticles (AgNPs) as substrates and is based on suspending the droplet of a mixture containing AgNPs and proteins from a hydrophobic surface. Drying the droplet at this suspended configuration allows the accumulation and packing of AgNPs and protein molecules in the middle of the droplet area rather than getting jammed at the edges of drying droplets as the solvent evaporates. A detection limit of down to 0.05 μg mL(-1) for some of the model proteins used in this study is obtained with this simple approach. The advantage of this method is its simplicity and improved sensitivity over other approaches reported in the literature.
ISSN:0003-2654
1364-5528
DOI:10.1039/c2an16296b