Determination of rifampicin based on fluorescence quenching of GSH capped CdTe/ZnS QDs

• Published in: Journal of luminescence 2012-09, Vol.132 (9), p.2484-2488
• Main Authors: Liu, Zhengqing, Yin, Pengfei, Gong, Huiping, Li, Pingping, Wang, Xiaodan, He, Youqiu
• Format: Article
• Language: English
• Subjects: Cadmium tellurides; Calibration; Condensed matter: electronic structure, electrical, magnetic, and optical properties; Determination; Exact sciences and technology; Fluorescence; Fluorescence quenching; Luminescence; Optical properties and condensed-matter spectroscopy and other interactions of matter with particles and radiation; Optical properties of low-dimensional, mesoscopic, and nanoscale materials and structures; Optimization; Physics; Quantum dots; Quenching; Rifampicin; Wavelengths; Zinc sulfides
• ISSN: 0022-2313; 1872-7883
• DOI: 10.1016/j.jlumin.2012.03.072

Aqueous glutathione (GSH)-capped CdTe/ZnS QDs with the diameter of 3–4nm were synthesized. The fluorescence of CdTe/ZnS QDs at 577nm was quenched in the presence of rifampicin (Rfp), with excitation wavelength at 350nm. The mechanism of the interaction of CdTe/ZnS QDs with Rfp was investigated. Under the optimal conditions, the calibration plot of ln(F0/F) was linear in the range 0.83–56μgmL−1 with concentration of Rfp, and the detection limit was 0.25μgmL−1. The proposed method was successfully applied to the determination of Rfp in its commercial capsules, and satisfactory results were obtained. The recovery of the method was in the range 98.6–103.2%. ► Fluorescence of CdTe/ZnS QDs was found to be quenched notably by rifampicin. ► A simple, rapid and sensitive fluorimetric method for the determination of Rfp was developed. ► Interaction mechanism of CdTe/ZnS QDs with rifampicin was investigated. ► Proposed method was applied for analysis of rifampicin in capsules.