Development of a scaled-down aerobic fermentation model for scale-up in recombinant protein vaccine manufacturing

Highlights ► Inconsistencies in scale-up fermentation of biological products are demonstrated. ► Inconsistencies are attributed to different oxygen mass transfer coefficients ( k L a ). ► A method to “tune” the k L a to the same value at each fermentation scale is shown. ► Consistent fermentation pe...

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Bibliographic Details
Published in:Vaccine 2012-08, Vol.30 (38), p.5695-5698
Main Authors: Farrell, Patrick, Sun, Jacob, Gao, Meg, Sun, Hong, Pattara, Ben, Zeiser, Arno, D’Amore, Tony
Format: Article
Language:English
Subjects:
Aerobiosis
Allergy and Immunology
Bioreactors - microbiology
Biotechnology - methods
E coli
Electrodes
Escherichia coli
Escherichia coli - genetics
Escherichia coli - growth & development
Escherichia coli - metabolism
Fermentation
Glucose
Manufacturing
Mass transfer
Metabolism
Oxygen
Protein expression
Proteins
Recombinant protein
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Scale-down model
Technology, Pharmaceutical - methods
Vaccines
Vaccines, Synthetic - biosynthesis
Vaccines, Synthetic - genetics
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Summary:Highlights ► Inconsistencies in scale-up fermentation of biological products are demonstrated. ► Inconsistencies are attributed to different oxygen mass transfer coefficients ( k L a ). ► A method to “tune” the k L a to the same value at each fermentation scale is shown. ► Consistent fermentation performance at all scales results from k L a tuning. ► An empirical correlation for k L a is used as a tool to extrapolate to larger scales.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2012.05.031