Isolation and characterization of a harvest-induced promoter of an alfalfa gene, hi7

A cDNA library was made by subtractive hybridization of cDNA from non-harvested and harvested alfalfa plants. One cDNA clone, hi7 , in Northern blot analysis showed a high level of transcription of this gene in harvested tissue, but not in non-harvested tissue, nor in wounded or heat shock treated t...

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Bibliographic Details
Published in:Plant growth regulation 2011-06, Vol.64 (2), p.119-128
Main Authors: Zhang, Jian, Yu, Wen-Jin, Xiong, Ai-Sheng, Bahramnejad, Bahman, Erickson, Larry R.
Format: Article
Language:English
Subjects:
Agriculture
Alfalfa
Biomedical and Life Sciences
Callus
genomics
GUS gene
Harvesting
Heat shock
Leaves
Life Sciences
Medicago truncatula
Original Paper
Plant Anatomy/Development
Plant Physiology
Plant Sciences
Plant tissues
Promoters
Temperature effects
Tobacco
Transcription
Transgenic plants
Walking
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Summary:A cDNA library was made by subtractive hybridization of cDNA from non-harvested and harvested alfalfa plants. One cDNA clone, hi7 , in Northern blot analysis showed a high level of transcription of this gene in harvested tissue, but not in non-harvested tissue, nor in wounded or heat shock treated tissue. A 640 bp promoter region isolated by genomic walking based on hi7 cDNA sequence, was fused to a GUS gene and transferred to tobacco and Medicago truncatula . When leaf tissue from transgenic tobacco plants was harvested, incubated for 24 h at room temperature, and then stained for GUS activity, a strong blue colour was observed throughout the tissue, but not in tissue that was stained immediately following harvesting. The expression of the GUS gene was also induced in tobacco plants sprayed with abscissic acid (ABA) and in callus derived from these plants when transferred to medium containing ABA.
ISSN:0167-6903
1573-5087
DOI:10.1007/s10725-010-9546-x