Biological, immunological and regenerative characteristics of placenta-derived mesenchymal stem cell isolated using a time-gradient attachment method

It has been verified that placenta contains multi-lineage mesenchymal stem cells (MSCs). We have used a time-gradient attachment method to isolate placenta-derived MSCs (PMSCs). The morphology, differentiation potential, immunogenicity and xenogenic reconstruction potential of these PMSCs were exami...

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Bibliographic Details
Published in:Stem cell research 2012-09, Vol.9 (2), p.110-123
Main Authors: Yuan, Wenji, Zong, Chen, Huang, Yingzhi, Gao, Ying, Shi, Dongyan, Chen, Changsong, Liu, Liyue, Wang, Jinfu
Format: Article
Language:English
Subjects:
Animals
Calvaria
Cell Adhesion
Cell Differentiation
Cell Proliferation
Cell Separation - methods
Cell Shape
Differentiation
Female
Histocompatibility antigen HLA
Humans
Immunogenicity
Immunomodulation
Lymphocytes T
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - immunology
Mesenchyme
Mixed leukocyte reaction
Phenotype
Placenta
Placenta - cytology
Pregnancy
Rats
Rats, Sprague-Dawley
Regeneration - physiology
Skull - pathology
Stem cells
Therapeutic applications
Time Factors
Wound Healing
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Summary:It has been verified that placenta contains multi-lineage mesenchymal stem cells (MSCs). We have used a time-gradient attachment method to isolate placenta-derived MSCs (PMSCs). The morphology, differentiation potential, immunogenicity and xenogenic reconstruction potential of these PMSCs were examined. The results showed that PMSCs isolated using the time-gradient attachment method showed higher potential of in vitro proliferation and multi-lineage differentiation. PMSCs isolated using the time-gradient attachment method showed a low immunogenicity. HLA-A gene fragment and no HLA-DR gene fragment were detected in PMSCs isolated using the time-gradient attachment method, and the mixed lymphocyte reaction (MLR) assay identified that these cells inhibited the proliferation of the allogeneic T-lymphocytes induced by PHA. The transplantation in calvaria of rats showed that PMSCs had the higher xenogenic reconstruction potential. Finally, the significance of PMSCs isolated using the time-gradient attachment method in experimental and clinical applications is discussed. ► A time-gradient attachment method was used to purify placenta-derived MSCs (PMSCs). ► Purified PMSCs had the potentials of proliferation and multi-differentiation. ► Purified PMSCs inhibited the proliferation of the allogeneic T-lymphocytes. ► Purified PMSCs had the xenogenic bone-reconstruction potential in rats.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2012.05.003