mRNA profiles of piRNA pathway genes in emerald ash borer Agrilus planipennis

Abstract Recent advances of Piwi-interacting RNAs （piRNAs） have enabled a better understanding of their putative roles in the protection of gerrn cells against selfish genetic elements. The germ plasm proteins namely, PIWI and VASA, are key players in regulation of piRNAs. However, the biogenesis of...

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Bibliographic Details
Published in:Insect science 2012-08, Vol.19 (4), p.455-460
Main Authors: Mittapalli, Omprakash, Mamidala, Praveen, Rajarapu, Swapna Priya
Format: Article
Language:English
Subjects:
Agrilus
Agrilus planipennis
Developmental stages
Germ cells
Homology
mRNA
Ovaries
Pests
piRNA
PIWI
Transcription
VASA
vasa gene
vasa protein
发育阶段
基因
氨基酸序列
白蜡窄吉丁
组织测定
蛋白相互作用
配置文件
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Summary:Abstract Recent advances of Piwi-interacting RNAs （piRNAs） have enabled a better understanding of their putative roles in the protection of gerrn cells against selfish genetic elements. The germ plasm proteins namely, PIWI and VASA, are key players in regulation of piRNAs. However, the biogenesis of piRNAs remains poorly understood. We report the characterization and the transcript profiles of a PIWI and VASA gene in various tissues and developmental stages of the emerald ash borer （Agrilusplanipennis）, an exotic invasive insect pest that has attained a high impact status worldwide. The deduced amino acid sequences of both these genes （herein referred to ＂ApPIWI and ApVASA＂） revealed homology with similar proteins from other insect and non-insect taxa. Peak transcript levels for ApPIWI and ApVASA were observed in the ovaries （compared to the other tissues assayed） and in adults （compared to the other developmental stages assayed）. To our knowledge this is the first report of profiling piRNA pathway genes in A. planipennis. These results provide new insights into the putative function of the PIWI and VASA proteins in insects.
ISSN:1672-9609
1744-7917
DOI:10.1111/j.1744-7917.2011.01466.x