Stepping towards highly flexible aptamers: enzymatic recognition studies of unlocked nucleic acid nucleotides

Enzymatic recognition of unlocked nucleic acid (UNA) nucleotides was successfully accomplished. Therminator DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of a 81 mer UNA-modified DNA library was efficiently achieved by...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2012-01, Vol.48 (44), p.5503-5505
Main Authors: Dubois, Camille, Campbell, Meghan A, Edwards, Stacey L, Wengel, Jesper, Veedu, Rakesh N
Format: Article
Language:English
Subjects:
Aptamers
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - metabolism
Base Sequence
DNA Primers - chemistry
DNA Primers - genetics
DNA-Directed DNA Polymerase - chemistry
DNA-Directed DNA Polymerase - genetics
DNA-Directed DNA Polymerase - metabolism
Gene Library
Molecular Sequence Data
Nucleic Acid Conformation
Polymerase Chain Reaction - methods
Substrate Specificity
Thermococcus - chemistry
Thermococcus - enzymology
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Summary:Enzymatic recognition of unlocked nucleic acid (UNA) nucleotides was successfully accomplished. Therminator DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of a 81 mer UNA-modified DNA library was efficiently achieved by KOD DNA polymerase.
ISSN:1359-7345
1364-548X
DOI:10.1039/c2cc31316b