A Study of Peripheral Blood in Hedgehogs in Turkey

The aim of this study was to determine diameters of blood cells, differential counts of peripheral blood leukocytes, alpha-naphthyl acetate esterase (ANAE), acid phosphatase (ACP-ase) activity of some leukocyte types, and enzymatic positivity percentages of peripheral blood lymphocytes in two hedgeh...

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Bibliographic Details
Published in:Journal of zoo and wildlife medicine 2011-09, Vol.42 (3), p.392-398
Main Authors: Özparlak, Haluk, Çelik, Ilhami, Sur, Emrah, Özaydin, Tuğba, Arslan, Atilla
Format: Article
Language:English
Subjects:
Acetates
acid phosphatase
alpha-naphthyl acetate esterase
Animals
Blood
Blood cells
Blood Platelets - cytology
Erinaceus concolor
Erythrocytes
Erythrocytes - cytology
Hedgehogs
Hedgehogs - blood
hematology
Hemiechinus auritus
Leukocyte Count - veterinary
Leukocytes
Leukocytes - cytology
Lymphocytes
Monocytes
Naphthol AS D Esterase - blood
Neutrophils
T lymphocytes
Turkey
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Summary:The aim of this study was to determine diameters of blood cells, differential counts of peripheral blood leukocytes, alpha-naphthyl acetate esterase (ANAE), acid phosphatase (ACP-ase) activity of some leukocyte types, and enzymatic positivity percentages of peripheral blood lymphocytes in two hedgehogs species, Hemiechinus auritus, the long-eared hedgehog, and Erinaceus concolor, the southern white-breasted hedgehog. Air-dried peripheral blood smears were stained with May-Grünwald–Giemsa stain. ANAE and ACP-ase were stained in glutaraldehyde-acetone-fixed smears. ANAE-positive lymphocytes displayed a dot-like positivity pattern characterized with 1–5 reddish brown cytoplasmic granules, whereas ACP-ase positive lymphocytes displayed a dot-like positivity pattern characterized with 1–3 pinkish cytoplasmic granules. Monocytes gave a diffuse and strong reaction while neutrophils displayed a weak positive reaction for ANAE and ACP-ase. No difference was observed in mean diameters of peripheral blood cells of these species. It was found that lymphocytes made up the majority (64.3% and 65.5%) of leukocytes, followed by neutrophils (23.9% and 23.3%), eosinophils (9.0% and 7.6%), monocytes (1.8% and 2.3%), and basophils (1.0% and 1.3%) in H. auritus and E. concolor, respectively. Mean ANAE positivity of lymphocytes was 36.6% and 51.3% and ACP-ase positivity was 32.1% and 37.5% for H. auritus and E. concolor, respectively. The ANAE positivity of lymphocytes in E. concolor was significantly (P < 0.05) higher than that of H. auritus.
ISSN:1042-7260
1937-2825
DOI:10.1638/2010-0119.1