Chimeric flagellin as the self-adjuvanting antigen for the activation of immune response against Helicobacter pylori

Highlights ► Chimeric flagellin was composed of E. coli FliC and H. pylori FlaA domains. ► Chimeric flagellin activates innate immune response through TLR5 activation. ► Variable domains of H. pylori FlaA represent the immunodominant epitopes. ► Chimeric flagellin acted as adjuvant eliciting strong...

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Bibliographic Details
Published in:Vaccine 2012-08, Vol.30 (40), p.5856-5863
Main Authors: Mori, Jerneja, Vranac, Tanja, Smrekar, Boštjan, Černilec, Maja, Šerbec, Vladka Čurin, Horvat, Simon, Ihan, Alojz, Benčina, Mojca, Jerala, Roman
Format: Article
Language:English
Subjects:
Adaptive Immunity
Adjuvant
Allergy and Immunology
Animals
Antibiotics
antibodies
Antibodies, Bacterial - blood
antigens
Antigens, Bacterial - immunology
bacteria
Bacterial Vaccines - immunology
blood serum
Chimeric protein
chimerism
Colonization
Duodenum
engineering
Escherichia coli
Escherichia coli - immunology
FlaA protein
Flagella
Flagellin
Flagellin - immunology
flagellum
Gastric cancer
Gastritis
HEK293 Cells
Helicobacter Infections - immunology
Helicobacter Infections - prevention & control
Helicobacter pylori
Helicobacter pylori - immunology
Humans
Immune response
Immunity, Innate
Immunodominant Epitopes - immunology
Immunoglobulin A
Immunoglobulin A - blood
Immunoglobulin G
Immunoglobulin G - blood
Infection
Innate immunity
Mice
Mice, Inbred BALB C
peptic ulcers
Recombinant Fusion Proteins - immunology
stomach
stomach neoplasms
TLR5 protein
Toll-like receptor 5
Toll-Like Receptor 5 - immunology
Toll-like receptors
vaccination
Vaccines
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Description
Summary:Highlights ► Chimeric flagellin was composed of E. coli FliC and H. pylori FlaA domains. ► Chimeric flagellin activates innate immune response through TLR5 activation. ► Variable domains of H. pylori FlaA represent the immunodominant epitopes. ► Chimeric flagellin acted as adjuvant eliciting strong serum IgG antibody response. ► Chimeric flagellin with alum protects mice against H. pylori colonization.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2012.07.011