Melatonin prevents hypochlorous acid-induced alterations in microtubule and chromosomal structure in metaphase-II mouse oocytes

• Published in: Journal of pineal research 2012-09, Vol.53 (2), p.122-128
• Main Authors: Banerjee, Jashoman, Maitra, Dhiman, Diamond, Michael P., Abu-Soud, Husam M.
• Format: Article
• Language: English
• Subjects: Animals; Chromosomes - drug effects; Chromosomes - metabolism; endometriosis; Female; hypochlorous acid; Hypochlorous Acid - pharmacology; infertility; melatonin; Melatonin - pharmacology; Metaphase - drug effects; Metaphase - genetics; Mice; Microscopy, Confocal; Microscopy, Fluorescence; microtubule; Microtubules - drug effects; Microtubules - metabolism; myeloperoxidase; Oocytes - cytology; Oocytes - drug effects; Oocytes - metabolism; oxidative stress; pelvic inflammation; spindle apparatus
• ISSN: 0742-3098; 1600-079X
• DOI: 10.1111/j.1600-079X.2012.00977.x

:  Hypochlorous acid (HOCl) is generated by myeloperoxidase, using chloride and hydrogen peroxide as substrates. Here we demonstrate that HOCl alters metaphase‐II mouse oocyte microtubules and chromosomal (CH) alignment which can be prevented by melatonin. Metaphase‐II mouse oocytes, obtained commercially, were grouped as: control, melatonin (150, 200 nmol/mL), HOCl (10, 20, 50, and 100 nmol/mL), and HOCl (50 nmol/mL) pretreated with 150 and 200 nmol/mL of melatonin. Microtubule and CH alignment was studied utilizing an indirect immunofluorescence technique and scored by two observers. Pearson chi‐square test and Fisher’s exact test were used to compare outcomes between controls and treated groups and also among each group. Poor scores for the spindle and chromosomes increased significantly at 50 nmol/mL of HOCl (P