Involvement of SHP2 in focal adhesion, migration and differentiation of neural stem cells

Abstract Objectives SHP2 (Src-homology-2 domain-containing protein tyrosine phosphatase) plays an important role in cell adhesion, migration and cell signaling. However, its role in focal adhesion, differentiation and migration of neural stem cells is still unclear. Methods In this study, rat neuros...

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Bibliographic Details
Published in:Brain & development (Tokyo. 1979) 2012-09, Vol.34 (8), p.674-684
Main Authors: Huang, Yuahn-Sieh, Cheng, Cheng-Yi, Chueh, Sheau-Huei, Hueng, Dueng-Yuan, Huang, Yu-Fen, Chu, Chun-Ming, Wu, Sheng-Tang, Tai, Ming-Cheng, Liang, Chang-Min, Liao, Mei-Hsiu, Chen, Chia-Chieh, Shen, Lie-Hang, Ma, Kuo-Hsing
Format: Article
Language:English
Subjects:
Adhesion
Animals
Astrocytes
Axonogenesis
Blotting, Western
Cell Adhesion
Cell Differentiation
Cell migration
Cell Movement
Collagen
Differentiation
Extracellular signal-regulated kinase
Focal adhesion kinase
Focal Adhesions - metabolism
Glial cells
Immunohistochemistry
Migration
Neural stem cells
Neural Stem Cells - cytology
Neural Stem Cells - metabolism
Neurology
Neurons
neurospheres
Oligodendrocytes
paxillin
Phosphatase
Protein Tyrosine Phosphatase, Non-Receptor Type 11 - metabolism
Protein-tyrosine-phosphatase
Rats
Rats, Sprague-Dawley
RhoA protein
SHP2
Src protein
Thinning
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Summary:Abstract Objectives SHP2 (Src-homology-2 domain-containing protein tyrosine phosphatase) plays an important role in cell adhesion, migration and cell signaling. However, its role in focal adhesion, differentiation and migration of neural stem cells is still unclear. Methods In this study, rat neurospheres were cultured in suspension and differentiated neural stem cells were cultured on collagen-coated surfaces. Results The results showed that p-SHP2 co-localized with focal adhesion kinase (FAK) and paxillin in neurospheres and in differentiated neural precursor cells, astrocytes, neurons, and oligodendrocytes. Suppression of SHP2 activity by PTP4 or siRNA-mediated SHP2 silencing caused reduction in the cell migration and neurite outgrowth, and thinning of glial cell processes. Differentiation-induced activation of FAK, Src, paxillin, ERK1/2, and RhoA was decreased by SHP2 inactivation. Conclusions These results indicate that SHP2 is recruited in focal adhesions of neural stem cells and regulates focal adhesion formation. SHP2-mediated regulation of neural differentiation and migration may be related to formation of focal adhesions and RhoA and ERK1/2 activation.
ISSN:0387-7604
1872-7131
DOI:10.1016/j.braindev.2011.10.011