Anti-allergic effect of lactic acid bacteria isolated from seed mash used for brewing sake is not dependent on the total IgE levels

• Published in: Journal of bioscience and bioengineering 2012-09, Vol.114 (3), p.292-296
• Main Authors: Masuda, Yasuyuki, Takahashi, Toshinari, Yoshida, Kazutoshi, Nishitani, Yosuke, Mizuno, Masashi, Mizoguchi, Haruhiko
• Format: Article
• Language: English
• Subjects: Allergic diseases; animal models; Animals; Anti-allergic activity; Anti-Allergic Agents - immunology; Anti-Allergic Agents - metabolism; Atopic dermatitis; Biological and medical sciences; Biotechnology; Caco-2 Cells; Cell Line; dermatitis; Dermatitis, Atopic - immunology; Dermatitis, Atopic - microbiology; Disease Models, Animal; Ear - pathology; epithelial cells; Fundamental and applied biological sciences. Psychology; Humans; Hypersensitivity, Immediate - immunology; Hypersensitivity, Immediate - microbiology; IgE; IgE-mediated hypersensitivity reaction; immunoglobulin E; Immunoglobulin E - blood; Immunoglobulin E - immunology; Intestines - immunology; Intestines - microbiology; Lactic Acid - metabolism; Lactic acid bacteria; Lactobacillus; Lactobacillus - immunology; Lactobacillus - metabolism; Lactobacillus curvatus; Lactobacillus sakei; Leuconostoc - immunology; Leuconostoc - metabolism; Leuconostoc mesenteroides; macrophages; Macrophages - immunology; Macrophages - microbiology; mash; Mast Cells - immunology; Mast Cells - microbiology; Mice; sake; Seeds - microbiology; skin lesions; tumor necrosis factor-alpha; Tumor Necrosis Factor-alpha - biosynthesis; Tumor Necrosis Factor-alpha - immunology; Wine - microbiology
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2012.04.017

Lactic acid bacteria (LAB) in fermented foods have attracted considerable attention recently as treatment options for allergic diseases, the incidence of which has been increasing worldwide. Five strains of LAB isolated from kimoto, the traditional seed mash used for brewing sake, were screened for the ability to suppress IgE-mediated hypersensitivity reaction. Leuconostoc mesenteroides and Lactobacillus sakei, the normal microflora in kimoto, significantly suppressed the reaction, but the contaminant Lactobacillus curvatus did not. Next, we examined the effect of L. sakei LK-117 on atopic dermatitis in the NC/Nga mouse model. LK-117 supplementation significantly reduced the development of atopic dermatitis-like skin lesions in a manner independent of the IgE plasma levels. In the in vitro intestinal model constructed using the human intestinal epithelial cell line Caco-2 and murine macrophage cell line RAW 264.7, treatment with L. sakei LK-117, but not L. curvatus, significantly upregulated TNF-α production from RAW264.7 cells. This result indicated that L. sakei on the apical side affected the macrophages on the basolateral side, and this organism may have the ability to improve allergy symptoms mediated by the intestinal immune system.