MPN-PCR detection and antimicrobial resistance of Listeria monocytogenes isolated from raw and ready-to-eat foods in Malaysia

Listeria monocytogenes is a major food-borne pathogen with an opportunistic character that is able to cause severe human listeriosis worldwide. The surveillance and detection system concerning food safety in the country needs to be improved. Therefore, it was a primary aim of this study to provide a...

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Bibliographic Details
Published in:Food control 2012-12, Vol.28 (2), p.309-314
Main Authors: Marian, M.N., Sharifah Aminah, S.M., Zuraini, M.I., Son, R., Maimunah, M., Lee, H.Y., Wong, W.C., Elexson, N.
Format: Article
Language:English
Subjects:
Ampicillin
Antibiotic resistance
Hyla
Listeria monocytogenes
MPN-PCR
Ready-to-eat foods
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Summary:Listeria monocytogenes is a major food-borne pathogen with an opportunistic character that is able to cause severe human listeriosis worldwide. The surveillance and detection system concerning food safety in the country needs to be improved. Therefore, it was a primary aim of this study to provide a general baseline on the L. monocytogenes contamination in foods from local markets. A total of 140 raw and ready-to-eat food samples were analyzed by plating on selective PALCAM media and the suspected colonies were confirmed by MPN-PCR technique. Positive isolates were evaluated for their susceptibility to 8 commonly used antibiotic agents by using disc diffusion assay. The isolated strains yield PCR products of 938 bp 16S rRNA gene and 701 bp hylA virulence gene specific for L. monocytogenes. The microbial load of L. monocytogenes was found to range from
ISSN:0956-7135
1873-7129
DOI:10.1016/j.foodcont.2012.05.030