Proteomic analysis of the pinworm Syphacia muris (Nematoda: Oxyuridae), a parasite of laboratory rats

Abstract Syphacia muris (Nematoda: Oxyuridae) is a ubiquitous nematode that commonly infects rats in the laboratory which can interfere in the development of biological assays. The somatic extract of S. muris adults collected from infected rats was investigated using a proteomic approach. A shot-gun...

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Bibliographic Details
Published in:Parasitology international 2012-12, Vol.61 (4), p.561-564
Main Authors: Sotillo, Javier, Trelis, María, Cortés, Alba, Valero, María Luz, del Pino, Manuel Sánchez, Esteban, José Guillermo, Marcilla, Antonio, Toledo, Rafael
Format: Article
Language:English
Subjects:
adults
Animals
bioassays
biological development
calcium-binding proteins
cell cycle
computer software
enzymes
Gastroenterology and Hepatology
Gene Expression Regulation - physiology
Helminth Proteins - genetics
Helminth Proteins - metabolism
host-parasite relationships
Infectious Disease
Laboratory Animal Science
liquid chromatography
Nematoda
Nematode Infections - parasitology
Nematode Infections - veterinary
Oxyuridae
Oxyuroidea - genetics
Oxyuroidea - metabolism
parasites
parasitology
protein metabolism
proteome
Proteomics
Rats
Rats, Wistar
receptors
Rodent Diseases - parasitology
Rodents
Syphacia
Syphacia muris
tandem mass spectrometry
Transcriptome
transmembrane proteins
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Summary:Abstract Syphacia muris (Nematoda: Oxyuridae) is a ubiquitous nematode that commonly infects rats in the laboratory which can interfere in the development of biological assays. The somatic extract of S. muris adults collected from infected rats was investigated using a proteomic approach. A shot-gun liquid chromatography/tandem mass spectrometry procedure was used. We used the MASCOT search engine (Matrix-Science) and ProteinPilot software v2.0 (Applied Biosystems) for the database search. A total of 359 proteins were accurately identified from the worms. The largest protein families consisted of metabolic enzymes and those involved in the nucleic metabolism and cell cycle. Proteins of transmembrane receptors and those involved in protein metabolism, chaperones, structural and motor, signalling and calcium-binding proteins also were identified in the proteome of S. muris . Proteome array of S. muris may contribute to further elucidation of biological system of S. muris as well as host–parasite relationships.
ISSN:1383-5769
1873-0329
DOI:10.1016/j.parint.2012.05.004