Molecularly imprinted solid phase microextraction fiber for trace analysis of catecholamines in urine and serum samples by capillary electrophoresis

• Published in: Talanta (Oxford) 2012-09, Vol.99, p.270-276
• Main Authors: Zhang, Xinfeng, Xu, Shuxia, Lim, Jae-Min, Lee, Yong-Ill
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Blood Chemical Analysis - methods; Capillary electrophoresis; Catecholamines - blood; Catecholamines - urine; Chemistry; Chromatographic methods and physical methods associated with chromatography; Dapamine; Electrophoresis, Capillary - methods; Epinephrine; Exact sciences and technology; Humans; Molecular Imprinting - methods; Molecularly imprinted polymer fiber; Norepinephrine; Other chromatographic methods; Polymerization; Polymers - chemical synthesis; Polymers - chemistry; Reproducibility of Results; Solid Phase Microextraction - methods; Solid phase miroextraction; Solvents - chemistry; Temperature; Time Factors; Urinalysis - methods
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2012.05.050

A selective and flexible monolithic moleculary imprinted polymer (MIP) fiber was developed in batch for solid phase microextraction (SPME) of catecholamines (CAs), i.e., dopamine (DA), epinephrine (E) and norepinephrine (NE), and coupled with capillary electrophoresis (CE) for trace analysis of urine and serum samples. The polymer fiber was synthesized in-situ simply using a flexible capillary as a mold and the polymerization protocols and SPME experimental conditions were examined in detail. The reproducibility of fiber to fiber fabrication (n=5) was in range of 5.9–9.8% for three CAs. The fiber also shows high stability without any deterioration of extraction performance after 30 times use. Under the established optimum conditions, the limits of detection for DA, E, and NE were 7.4, 4.8, and 7.1nmolL−1, respectively, with the enhancement factor over 100 after MIP-SPME. The specific selectivity to three CAs was discovered with the developed MIP fibers compared with non-imprinted polymer (NIP) fiber. Finally, the MIP fibers were successfully applied for selective extraction of CAs in urine and serum samples with the relative recoveries ranging from 85% to 103%. The fabricated MIP-fibers were promising in preparation of biological samples in batch followed by CE–UV detection. ► A new selective and flexible monolithic molecularly imprinted polymer (MIP) fiber. ► Developed a simple synthetic method by using a flexible capillary as a mold. ► Highly selective extraction of trace catecholamines in urine and serum samples. ► Very lower detection limits were achieved in range of 0.8–1.2ngmL−1.