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Urine iodide determination by ion-pair reversed-phase high performance liquid chromatography and pulsed amperometric detection

A sensitive and specific ion-pair reversed-phase high performance liquid chromatography (HPLC) method for urinary iodine analysis is described. This method is based on pulsed amperometric detection (PAD) using a silver working electrode (HPLC–PAD), which improves peak shape, electrode stability as w...

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Bibliographic Details
Published in:Talanta (Oxford) 2012-09, Vol.99, p.532-537
Main Authors: Nguyen, Vo Thanh Phuong, Piersoel, Virginie, El Mahi, Tarik
Format: Article
Language:English
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Summary:A sensitive and specific ion-pair reversed-phase high performance liquid chromatography (HPLC) method for urinary iodine analysis is described. This method is based on pulsed amperometric detection (PAD) using a silver working electrode (HPLC–PAD), which improves peak shape, electrode stability as well as linearity and reproducibility. A two-step extraction process consisting of solid phase extraction (SPE) and liquid–liquid extraction with dichloromethane was added in order to improve sample purification which is essential with the use of PAD. Treated samples were eluted on a C18 column, using a phosphate buffer containing ion-pairing reagent tetrabutylammonium and 5% MeOH. The calibration standard curves were linear up to 500μg/L and within-run and between-run coefficients of variation (CVs) were
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2012.06.028