Investigation of RNA-Ligand Interactions by 19F NMR Spectroscopy Using Fluorinated Probes

Spy swap: The interaction between an unlabeled RNA and unlabeled ligands (red hexagon) can be monitored by 19F NMR spectroscopy using small fluorinated diamines (green star) as spy reporters (see scheme). This technique also enables the visualization of the conformational capture of a riboswitch by...

Full description

Saved in:
Bibliographic Details
Published in:Angewandte Chemie International Edition 2012-09, Vol.51 (38), p.9530-9534
Main Authors: Lombès, Thomas, Moumné, Roba, Larue, Valéry, Prost, Elise, Catala, Marjorie, Lecourt, Thomas, Dardel, Frédéric, Micouin, Laurent, Tisné, Carine
Format: Article
Language:English
Subjects:
19F NMR spectroscopy
aminoglycosides
Aminoglycosides - chemistry
Fluorescent Dyes - chemistry
Fluorine - chemistry
Framycetin - chemistry
Ligands
Magnetic Resonance Spectroscopy
Neomycin - chemistry
Paromomycin - chemistry
reporter ligands
riboswitches
RNA
RNA - chemistry
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Spy swap: The interaction between an unlabeled RNA and unlabeled ligands (red hexagon) can be monitored by 19F NMR spectroscopy using small fluorinated diamines (green star) as spy reporters (see scheme). This technique also enables the visualization of the conformational capture of a riboswitch by its ligand.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201204083