Investigation of RNA-Ligand Interactions by 19F NMR Spectroscopy Using Fluorinated Probes

Spy swap: The interaction between an unlabeled RNA and unlabeled ligands (red hexagon) can be monitored by 19F NMR spectroscopy using small fluorinated diamines (green star) as spy reporters (see scheme). This technique also enables the visualization of the conformational capture of a riboswitch by...

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Published in:Angewandte Chemie International Edition 2012-09, Vol.51 (38), p.9530-9534
Main Authors: Lombès, Thomas, Moumné, Roba, Larue, Valéry, Prost, Elise, Catala, Marjorie, Lecourt, Thomas, Dardel, Frédéric, Micouin, Laurent, Tisné, Carine
Format: Article
Language:English
Subjects:
19F NMR spectroscopy
aminoglycosides
Aminoglycosides - chemistry
Fluorescent Dyes - chemistry
Fluorine - chemistry
Framycetin - chemistry
Ligands
Magnetic Resonance Spectroscopy
Neomycin - chemistry
Paromomycin - chemistry
reporter ligands
riboswitches
RNA
RNA - chemistry
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container_end_page 9534
container_issue 38
container_start_page 9530
container_title Angewandte Chemie International Edition
container_volume 51
creator Lombès, Thomas
Moumné, Roba
Larue, Valéry
Prost, Elise
Catala, Marjorie
Lecourt, Thomas
Dardel, Frédéric
Micouin, Laurent
Tisné, Carine
description Spy swap: The interaction between an unlabeled RNA and unlabeled ligands (red hexagon) can be monitored by 19F NMR spectroscopy using small fluorinated diamines (green star) as spy reporters (see scheme). This technique also enables the visualization of the conformational capture of a riboswitch by its ligand.
doi_str_mv 10.1002/anie.201204083
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source Wiley-Blackwell Read & Publish Collection
subjects 19F NMR spectroscopy
aminoglycosides
Aminoglycosides - chemistry
Fluorescent Dyes - chemistry
Fluorine - chemistry
Framycetin - chemistry
Ligands
Magnetic Resonance Spectroscopy
Neomycin - chemistry
Paromomycin - chemistry
reporter ligands
riboswitches
RNA
RNA - chemistry
title Investigation of RNA-Ligand Interactions by 19F NMR Spectroscopy Using Fluorinated Probes
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