A label-free real time fluorometric assay for protease and inhibitor screening with a released heme

A label free continuous assay for protease activity and inhibitor screening has been developed. A protease (trypsin) could digest hemoglobin. Free heme molecules were released. Strong π-π stacking and hydrophobic interactions with the perylene probe resulted in efficient quenching of the probe'...

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Published in:Chemical communications (Cambridge, England) England), 2012-10, Vol.48 (81), p.10123-10125
Main Authors: Li, Wenying, Chen, Jian, Jiao, Huping, Zhang, Qingfeng, Zhou, Huipeng, Yu, Cong
Format: Article
Language:English
Subjects:
Animals
Cattle
Enzyme Assays - methods
Fluorescent Dyes - analysis
Fluorescent Dyes - metabolism
Heme - metabolism
Humans
Perylene - analysis
Perylene - metabolism
Sensitivity and Specificity
Spectrometry, Fluorescence - methods
Trypsin - metabolism
Trypsin Inhibitors - pharmacology
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cited_by cdi_FETCH-LOGICAL-c287t-a7481af5e9f86725765ff4975d06c3b46be978cfb4cb0d8def06ff8f7e298a1a3
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container_end_page 10125
container_issue 81
container_start_page 10123
container_title Chemical communications (Cambridge, England)
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creator Li, Wenying
Chen, Jian
Jiao, Huping
Zhang, Qingfeng
Zhou, Huipeng
Yu, Cong
description A label free continuous assay for protease activity and inhibitor screening has been developed. A protease (trypsin) could digest hemoglobin. Free heme molecules were released. Strong π-π stacking and hydrophobic interactions with the perylene probe resulted in efficient quenching of the probe's monomer fluorescence.
doi_str_mv 10.1039/c2cc35374a
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source Royal Society of Chemistry
subjects Animals
Cattle
Enzyme Assays - methods
Fluorescent Dyes - analysis
Fluorescent Dyes - metabolism
Heme - metabolism
Humans
Perylene - analysis
Perylene - metabolism
Sensitivity and Specificity
Spectrometry, Fluorescence - methods
Trypsin - metabolism
Trypsin Inhibitors - pharmacology
title A label-free real time fluorometric assay for protease and inhibitor screening with a released heme
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