The application of high resolution diffusion NMR to the analysis of manuka honey

► We report the novel use of DOSY NMR in the analysis of honey. ► We report the preliminary identification of new components in honey. ► We have used qNMR to quantify components in complex mixtures. ► The preliminary antimicrobial analysis of manuka honey suggests component synergy. The application...

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Bibliographic Details
Published in:Food chemistry 2012-12, Vol.135 (4), p.2879-2886
Main Authors: Gresley, Adam Le, Kenny, Jackie, Cassar, Claire, Kelly, Alison, Sinclair, Alex, Fielder, Mark D.
Format: Article
Language:English
Subjects:
Animals
Anti-bacterial
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Bacteria - drug effects
Bacterial Infections - microbiology
Bees
Biological and medical sciences
Confectionery products and chocolate industries, honey
Diffusion NMR
Food industries
Fundamental and applied biological sciences. Psychology
Honey - analysis
Humans
Leptospermum - chemistry
Magnetic Resonance Spectroscopy - instrumentation
Magnetic Resonance Spectroscopy - methods
Manuka honey
Methylglyoxal
Molecular Structure
Phenyllactic acid
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Summary:► We report the novel use of DOSY NMR in the analysis of honey. ► We report the preliminary identification of new components in honey. ► We have used qNMR to quantify components in complex mixtures. ► The preliminary antimicrobial analysis of manuka honey suggests component synergy. The application of DOSY (Diffusion Ordered SpectroscopY) NMR as a technique for the virtual separation of key components of manuka honey and the implications for future discriminatory analysis of honey types is reported for the first time. The scope and the limitations of DOSY NMR are considered using the recently conceived DOSY Tool Box processing software and preliminary anti-bacterial data for the different honey types is reported.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2012.07.072