Plasma rich in growth factors (PRGF-Endoret) stimulates tendon and synovial fibroblasts migration and improves the biological properties of hyaluronic acid

• Published in: Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA sports traumatology, arthroscopy : official journal of the ESSKA, 2012-09, Vol.20 (9), p.1657-1665
• Main Authors: Anitua, E., Sanchez, M., De la Fuente, M., Zalduendo, M. M., Orive, G.
• Format: Article
• Language: English
• Subjects: Biomedical materials; Biotechnology; Blood; Blood platelets; Cell adhesion & migration; Cell Movement; Cells, Cultured; Experimental Study; Fibroblasts; Fibroblasts - physiology; Humans; Hyaluronic acid; Insulin-like growth factors; Intercellular Signaling Peptides and Proteins - pharmacology; Knees; Leukocytes; Medicine; Medicine & Public Health; Motility; Orthopedics; Plasma; Recovery; Regeneration - physiology; Sports; Surgery; Synovial Membrane - cytology; Technology; Tendons; Tendons - cytology; Tendons - physiology; Tissue engineering; Vascular endothelial growth factor; Wound healing; Wound Healing - physiology; Wounds
• ISSN: 0942-2056; 1433-7347
• DOI: 10.1007/s00167-011-1697-4

Purpose Cell migration plays an essential role in development, wound healing, and tissue regeneration. Plasma rich in growth factors (PRGF-Endoret) technology offers a potential source of growth factors involved in tissue regeneration. Here, we evaluate the potential of PRGF-Endoret over tendon cells and synovial fibroblasts migration and study whether the combination of this autologous technology with hyaluronic acid (HA) improves the effect and potential of the biomaterials over the motility of both types of fibroblasts. Methods Migration of primary tendon cells and synovial fibroblasts after culturing with either PRGF or PPGF (plasma poor in growth factors) at different doses was evaluated. Furthermore, the migratory capacity induced by the combination of PPGF and PRGF with HA was tested. Results PPGF stimulated migration of both types of cells but this effect was significantly higher when PRGF was used. Tendon cells showed an increase of 212% in migratory ability when HA was combined with PPGF and of 335% in the case of HA + PRGF treatment compared with HA alone. Conclusions PRGF-Endoret stimulates migration of tendon cells and synovial fibroblasts and improves the biological properties of HA.