A genetically-encoded photosensitiser demonstrates killing of bacteria by purely endogenous singlet oxygen

TagRFP, a fluorescent protein capable of photosensitizing the production of singlet oxygen, was expressed in E. coli . Subsequent exposure to green light induced bacterial cell death in a light-dose dependent manner. It is demonstrated for the first time that intracellular singlet oxygen is sufficie...

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Bibliographic Details
Published in:Photochemical & photobiological sciences 2012-09, Vol.11 (9), p.1411-1413
Main Authors: Ruiz-González, Rubén, White, John H., Agut, Montserrat, Nonell, Santi, Flors, Cristina
Format: Article
Language:English
Subjects:
Biochemistry
Biomaterials
Cell death
Chemistry
Communication
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - metabolism
Escherichia coli - radiation effects
Light
Luminescent Proteins - chemistry
Luminescent Proteins - genetics
Luminescent Proteins - metabolism
Oxygen
Photosensitization
Photosensitizing Agents - pharmacology
Physical Chemistry
Plant Sciences
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Singlet Oxygen - metabolism
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Summary:TagRFP, a fluorescent protein capable of photosensitizing the production of singlet oxygen, was expressed in E. coli . Subsequent exposure to green light induced bacterial cell death in a light-dose dependent manner. It is demonstrated for the first time that intracellular singlet oxygen is sufficient to kill bacteria.
ISSN:1474-905X
1474-9092
DOI:10.1039/c2pp25126d