Dietary Lysine Imbalance Affects Muscle Proteome in Zebrafish (Danio rerio): A Comparative 2D-DIGE Study

• Published in: Marine biotechnology (New York, N.Y.) N.Y.), 2012-10, Vol.14 (5), p.643-654
• Main Authors: de Vareilles, Mahaut, Conceição, Luis E. C, Gómez-Requeni, Pedro, Kousoulaki, Katerina, Richard, Nadège, Rodrigues, Pedro M, Fladmark, Kari E, Rønnestad, Ivar
• Format: Article
• Language: English
• Subjects: Accretion; adenosine triphosphate; Amino acids; Amino Acids - analysis; Animal Feed - analysis; animal growth; Animals; apolipoprotein A-I; Aquaculture; Biology; Biomedical and Life Sciences; Danio rerio; Diet; Dietary Supplements; Electrophoresis, Gel, Two-Dimensional - methods; Electrophoresis, Gel, Two-Dimensional - veterinary; Energy conservation; Energy storage; Engineering; fish; Fish feeds; Freshwater; Freshwater & Marine Ecology; H-transporting ATP synthase; Image Processing, Computer-Assisted; juveniles; Life Sciences; lysine; Lysine - administration & dosage; Lysine - pharmacology; Marine; Mass spectrometry; Metabolism; Microbiology; muscle protein; Muscle, Skeletal - drug effects; Muscle, Skeletal - metabolism; Musculoskeletal system; myofibrillar proteins; myosin light chains; Nutrition research; Original Article; Protein expression; protein sources; Protein synthesis; Proteins; proteome; Proteome - drug effects; Proteome - metabolism; Proteomics; Raw materials; Scientific imaging; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - veterinary; Studies; vegetable protein; Zebrafish; Zebrafish - metabolism; Zoology
• ISSN: 1436-2228; 1436-2236
• DOI: 10.1007/s10126-012-9462-3

Lysine (Lys) is an indispensable amino acid (AA) and generally the first limiting AA in vegetable protein sources in fish feeds. Inadequate dietary Lys availability may limit protein synthesis, accretion and growth of fish. This experiment aimed to further elucidate the role of Lys imbalance on growth by examining the myotomal muscle proteome of juvenile zebrafish (Danio rerio). Quadruplicate groups of 8 fish were fed either a low-Lys [Lys(−), 1.34 g kg−1], medium/control (Lys, 2.47 g kg−1) or high-Lys [Lys(+), 4.63 g kg−1] diet. Fish growth was monitored from 33 to 49 days post-fertilization (dpf) and trunk myotomal muscle proteome of Lys(−) and Lys(+) treatments were screened by 2D-DIGE and MALDI ToF tandem mass spectrometry. Growth rate was negatively affected by diet Lys(−). Out of 527 ± 11 (mean ± S.E.M.) protein spots detected (∼10–150 kDa and 4–7 pI value), 30 were over-expressed and 22 under-expressed in Lys(−) fish (|fold-change| >1.2, p value