The second transcribed spacer rDNA sequence: an effective genetic marker for inter-species phylogenetic analysis of trematodes in the order Strigeata

In the present study, the second nuclear internal transcribed spacer (ITS-2) rDNA of Schistosoma japonicum isolates in mainland China was amplified, sequenced, and assessed for inferring the intra- and inter-species phylogenetic relationships of trematodes in the order Strigeata. The fragment contai...

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Bibliographic Details
Published in:Parasitology research (1987) 2012-10, Vol.111 (4), p.1467-1472
Main Authors: Zhao, G. H., Li, J., Mo, X. H., Li, X. Y., Lin, R. Q., Zou, F. C., Weng, Y. B., Song, H. Q., Zhu, X. Q.
Format: Article
Language:English
Subjects:
Analysis
Animals
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
China
Cladistic analysis
DNA, Helminth - chemistry
DNA, Helminth - genetics
DNA, Ribosomal Spacer - chemistry
DNA, Ribosomal Spacer - genetics
Female
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Genetic Markers
Humans
Immunology
Invertebrates
Male
Medical Microbiology
Microbiology
Molecular Sequence Data
Nemathelminthia. Plathelmintha
Original Paper
Phylogeny
Polymorphism, Genetic
Schistosoma japonicum - classification
Schistosoma japonicum - genetics
Schistosomiasis japonica - parasitology
Sequence Analysis, DNA
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Summary:In the present study, the second nuclear internal transcribed spacer (ITS-2) rDNA of Schistosoma japonicum isolates in mainland China was amplified, sequenced, and assessed for inferring the intra- and inter-species phylogenetic relationships of trematodes in the order Strigeata. The fragment containing ITS-2 rDNA was obtained from 24 S. japonicum isolates from eight epidemic provinces in mainland China. The length polymorphisms were observed among these ITS-2 rDNA sequences, ranging from 343 to 346 bp, and the intra- and inter-population variations in ITS-2 sequence were 0.0–2.1 % among S. japonicum isolates in China. Phylogenetic analyses using the maximum parsimony and maximum likelihood methods revealed that the ITS-2 rDNA sequence is not a suitable marker for studying inter- and intra-population variation in S. japonicum . However, phylogenetic analysis of trematodes in the order Strigeata indicated that the ITS-2 rDNA sequence provides an effective molecular marker for studying inter-species phylogenetic relationships among trematodes in this order.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-012-2981-z