Indirect spectrophotometric determination of ascorbic acid with ferrozine by flow-injection analysis

A FIA indirect spectrophotometric determination of ascorbic acid was developed using its reducing action on Fe(III) in acidic medium and following the spectrophotometric determination of the reduced iron by using sodium 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4″-disulphonate (ferrozine) as chro...

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Bibliographic Details
Published in:Talanta (Oxford) 1998-11, Vol.47 (3), p.531-536
Main Authors: Molina-Dı́az, A, Ortega-Carmona, I, Pascual-Reguera, M.I
Format: Article
Language:English
Subjects:
Analysis
Ascorbic acid
Biological and medical sciences
Ferrozine
Fruit juices
General pharmacology
Medical sciences
Pharmaceutical preparations
Pharmacology. Drug treatments
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Summary:A FIA indirect spectrophotometric determination of ascorbic acid was developed using its reducing action on Fe(III) in acidic medium and following the spectrophotometric determination of the reduced iron by using sodium 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4″-disulphonate (ferrozine) as chromogenic reagent in buffered medium (pH 5.5) and monitoring the absorbance signal at 562 nm. A three-line manifold with two reaction coils was used: in the first reaction coil, ascorbic acid reduces Fe(III) to Fe(II); and in the second one, the complexation reaction is developed. The linear range of the method was 0.5–10 μg ml −1 of ascorbic acid, the detection limit being 0.028 μg ml −1. The proposed method was sensitive, rapid (sampling rate of 90 samples h −1) and reproducible (RSD 0.19%, n=10). Satisfactory results were obtained in the determination of ascorbic acid in pharmaceutical preparations, fruit juices and urine testifying the applicability of the method to real samples.
ISSN:0039-9140
1873-3573
DOI:10.1016/S0039-9140(98)00085-X